A set of 156 SNP markers for teak (Tectona grandis Linn. f.)

Novel single nucleotide polymorphism loci were identified for teak ( Tectona grandis Linn. f.) using a combined Next Generation Sequencing approach on the Illumina MiSeq system with genotyping on the Agena MassARRAY iPLEX™ platform. Three hundred and forty four putative loci were identified through sequencing of DNA fragments following a modified double digest Restriction Associated DNA Sequencing method. One hundred and eighty nine loci successfully amplified in 172 individuals from 20 populations in Myanmar. A total of 33 loci were removed from the final panel as two showed evidence of deviation from Hardy–Weinberg equilibrium, 17 were significantly linked and 23 were non-polymorphic. For the remaining 156 loci, expected heterozygosity ranged from 0.041 to 0.456 and F ST from 0.062 to 0.632. These genetic resources will prove useful for future studies into the population genetics and phylogeography of this important and iconic timber species.