Determination of the effectiveness of UV radiation as a means of disinfection of metalworking fluids
Microbial contamination of metalworking fluids (MWFs) causes biofouling and degradation and is also associated with several health hazards. Development of an effective control method is therefore essential to reduce microbial loading in MWFs. The present study investigated the efficacy and rapidity...
Gespeichert in:
Veröffentlicht in: | Annals of microbiology 2014, Vol.64 (2), p.831-838 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 838 |
---|---|
container_issue | 2 |
container_start_page | 831 |
container_title | Annals of microbiology |
container_volume | 64 |
creator | Saha, Ratul Donofrio, Robert S Bagley, Susan T |
description | Microbial contamination of metalworking fluids (MWFs) causes biofouling and degradation and is also associated with several health hazards. Development of an effective control method is therefore essential to reduce microbial loading in MWFs. The present study investigated the efficacy and rapidity of UV radiation as a means of disinfection of MWFs under laboratory conditions to determine parameters that could be used to design an in-line UV reactor for enclosed machines. High and low concentrations (10⁴–10⁷ CFU/mL) of three indicator bacteria, Pseudomonas fluorescens, P. oleovorans subsp. lubricantis and Mycobacterium chelonae, were evaluated both as pure cultures and in combinations. The target organisms were irradiated with a high intensity (192 μW/cm², 55 W) UV lamp for different exposure time under both static and mixed conditions. For these Pseudomonas species with high concentrations of cells under static conditions, only a 56 % reduction was observed within 10 min of exposure, whereas under mixed condition, a 99 % reduction was achieved within 2 min of exposure. In contrast only 74 % reduction was observed for M. chelonae. However, with low concentrations of cells under mixed conditions, a 99.99 % and 82 % reduction in viable count was observed for the Pseudomonas sp. and M. chelonae, respectively. Similar results were observed for mixed culture combinations. Based on these observations high intensity UV in combination with mixing could be successfully used as a means of disinfection of MWFs within a short exposure time and the parameters obtained from the study could be implemented to design a plug flow UV reactor. |
doi_str_mv | 10.1007/s13213-013-0722-x |
format | Article |
fullrecord | <record><control><sourceid>proquest_C6C</sourceid><recordid>TN_cdi_proquest_journals_2404397648</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2404397648</sourcerecordid><originalsourceid>FETCH-LOGICAL-c383t-fb57704fa1143afc2e8c0602d22b23d7acd490d062195df0cadefe253e86b8db3</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS0EEqXwAayIxDowfsRJlqg8JSQWULaWE4-LS-MUO4Xy9yQNEjsWI1sz58xIl5BTChcUIL-MlDPKUxgqZyzd7pEJLWSZMhBiv_9nJaSCSXpIjmJcAshSlGJCzDV2GBrndedan7Q26d4wQWux7twneoxxaM5fk6CNGyEdE500qP1uZFx0foePfoOdXn214d35RWJXG2fiMTmwehXx5PedkvntzcvsPn18unuYXT2mNS94l9oqy3MQVlMquLY1w6IGCcwwVjFucl0bUYIByWiZGQu1NmiRZRwLWRWm4lNyPu5dh_Zjg7FTy3YTfH9SMQGCl7kURU_RkapDG2NAq9bBNTp8KwpqCFONYSoYqg9TbXuHjU7sWb_A8Lf5P-lslKxulV4EF9X8mQGVAEChhJz_ADBQgiA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2404397648</pqid></control><display><type>article</type><title>Determination of the effectiveness of UV radiation as a means of disinfection of metalworking fluids</title><source>Springer Nature OA Free Journals</source><creator>Saha, Ratul ; Donofrio, Robert S ; Bagley, Susan T</creator><creatorcontrib>Saha, Ratul ; Donofrio, Robert S ; Bagley, Susan T</creatorcontrib><description>Microbial contamination of metalworking fluids (MWFs) causes biofouling and degradation and is also associated with several health hazards. Development of an effective control method is therefore essential to reduce microbial loading in MWFs. The present study investigated the efficacy and rapidity of UV radiation as a means of disinfection of MWFs under laboratory conditions to determine parameters that could be used to design an in-line UV reactor for enclosed machines. High and low concentrations (10⁴–10⁷ CFU/mL) of three indicator bacteria, Pseudomonas fluorescens, P. oleovorans subsp. lubricantis and Mycobacterium chelonae, were evaluated both as pure cultures and in combinations. The target organisms were irradiated with a high intensity (192 μW/cm², 55 W) UV lamp for different exposure time under both static and mixed conditions. For these Pseudomonas species with high concentrations of cells under static conditions, only a 56 % reduction was observed within 10 min of exposure, whereas under mixed condition, a 99 % reduction was achieved within 2 min of exposure. In contrast only 74 % reduction was observed for M. chelonae. However, with low concentrations of cells under mixed conditions, a 99.99 % and 82 % reduction in viable count was observed for the Pseudomonas sp. and M. chelonae, respectively. Similar results were observed for mixed culture combinations. Based on these observations high intensity UV in combination with mixing could be successfully used as a means of disinfection of MWFs within a short exposure time and the parameters obtained from the study could be implemented to design a plug flow UV reactor.</description><identifier>ISSN: 1590-4261</identifier><identifier>EISSN: 1869-2044</identifier><identifier>DOI: 10.1007/s13213-013-0722-x</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Applied Microbiology ; Biodegradation ; Biofouling ; Biomedical and Life Sciences ; Cell culture ; control methods ; Disinfection ; exposure duration ; health hazards ; indicator species ; Life Sciences ; lubricants ; Medical Microbiology ; Microbial contamination ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mixed culture ; mixing ; Mycobacterium chelonae ; Mycology ; Original Article ; Pseudomonas ; Pseudomonas fluorescens ; reduction ; Ultraviolet radiation</subject><ispartof>Annals of microbiology, 2014, Vol.64 (2), p.831-838</ispartof><rights>Springer-Verlag Berlin Heidelberg and the University of Milan 2013</rights><rights>Springer-Verlag Berlin Heidelberg and the University of Milan 2013.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-fb57704fa1143afc2e8c0602d22b23d7acd490d062195df0cadefe253e86b8db3</citedby><cites>FETCH-LOGICAL-c383t-fb57704fa1143afc2e8c0602d22b23d7acd490d062195df0cadefe253e86b8db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13213-013-0722-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13213-013-0722-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41101,41469,42170,42538,51300,51557</link.rule.ids><linktorsrc>$$Uhttps://doi.org/10.1007/s13213-013-0722-x$$EView_record_in_Springer_Nature$$FView_record_in_$$GSpringer_Nature</linktorsrc></links><search><creatorcontrib>Saha, Ratul</creatorcontrib><creatorcontrib>Donofrio, Robert S</creatorcontrib><creatorcontrib>Bagley, Susan T</creatorcontrib><title>Determination of the effectiveness of UV radiation as a means of disinfection of metalworking fluids</title><title>Annals of microbiology</title><addtitle>Ann Microbiol</addtitle><description>Microbial contamination of metalworking fluids (MWFs) causes biofouling and degradation and is also associated with several health hazards. Development of an effective control method is therefore essential to reduce microbial loading in MWFs. The present study investigated the efficacy and rapidity of UV radiation as a means of disinfection of MWFs under laboratory conditions to determine parameters that could be used to design an in-line UV reactor for enclosed machines. High and low concentrations (10⁴–10⁷ CFU/mL) of three indicator bacteria, Pseudomonas fluorescens, P. oleovorans subsp. lubricantis and Mycobacterium chelonae, were evaluated both as pure cultures and in combinations. The target organisms were irradiated with a high intensity (192 μW/cm², 55 W) UV lamp for different exposure time under both static and mixed conditions. For these Pseudomonas species with high concentrations of cells under static conditions, only a 56 % reduction was observed within 10 min of exposure, whereas under mixed condition, a 99 % reduction was achieved within 2 min of exposure. In contrast only 74 % reduction was observed for M. chelonae. However, with low concentrations of cells under mixed conditions, a 99.99 % and 82 % reduction in viable count was observed for the Pseudomonas sp. and M. chelonae, respectively. Similar results were observed for mixed culture combinations. Based on these observations high intensity UV in combination with mixing could be successfully used as a means of disinfection of MWFs within a short exposure time and the parameters obtained from the study could be implemented to design a plug flow UV reactor.</description><subject>Applied Microbiology</subject><subject>Biodegradation</subject><subject>Biofouling</subject><subject>Biomedical and Life Sciences</subject><subject>Cell culture</subject><subject>control methods</subject><subject>Disinfection</subject><subject>exposure duration</subject><subject>health hazards</subject><subject>indicator species</subject><subject>Life Sciences</subject><subject>lubricants</subject><subject>Medical Microbiology</subject><subject>Microbial contamination</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mixed culture</subject><subject>mixing</subject><subject>Mycobacterium chelonae</subject><subject>Mycology</subject><subject>Original Article</subject><subject>Pseudomonas</subject><subject>Pseudomonas fluorescens</subject><subject>reduction</subject><subject>Ultraviolet radiation</subject><issn>1590-4261</issn><issn>1869-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EEqXwAayIxDowfsRJlqg8JSQWULaWE4-LS-MUO4Xy9yQNEjsWI1sz58xIl5BTChcUIL-MlDPKUxgqZyzd7pEJLWSZMhBiv_9nJaSCSXpIjmJcAshSlGJCzDV2GBrndedan7Q26d4wQWux7twneoxxaM5fk6CNGyEdE500qP1uZFx0foePfoOdXn214d35RWJXG2fiMTmwehXx5PedkvntzcvsPn18unuYXT2mNS94l9oqy3MQVlMquLY1w6IGCcwwVjFucl0bUYIByWiZGQu1NmiRZRwLWRWm4lNyPu5dh_Zjg7FTy3YTfH9SMQGCl7kURU_RkapDG2NAq9bBNTp8KwpqCFONYSoYqg9TbXuHjU7sWb_A8Lf5P-lslKxulV4EF9X8mQGVAEChhJz_ADBQgiA</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Saha, Ratul</creator><creator>Donofrio, Robert S</creator><creator>Bagley, Susan T</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2014</creationdate><title>Determination of the effectiveness of UV radiation as a means of disinfection of metalworking fluids</title><author>Saha, Ratul ; Donofrio, Robert S ; Bagley, Susan T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-fb57704fa1143afc2e8c0602d22b23d7acd490d062195df0cadefe253e86b8db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Applied Microbiology</topic><topic>Biodegradation</topic><topic>Biofouling</topic><topic>Biomedical and Life Sciences</topic><topic>Cell culture</topic><topic>control methods</topic><topic>Disinfection</topic><topic>exposure duration</topic><topic>health hazards</topic><topic>indicator species</topic><topic>Life Sciences</topic><topic>lubricants</topic><topic>Medical Microbiology</topic><topic>Microbial contamination</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mixed culture</topic><topic>mixing</topic><topic>Mycobacterium chelonae</topic><topic>Mycology</topic><topic>Original Article</topic><topic>Pseudomonas</topic><topic>Pseudomonas fluorescens</topic><topic>reduction</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saha, Ratul</creatorcontrib><creatorcontrib>Donofrio, Robert S</creatorcontrib><creatorcontrib>Bagley, Susan T</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Annals of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Saha, Ratul</au><au>Donofrio, Robert S</au><au>Bagley, Susan T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of the effectiveness of UV radiation as a means of disinfection of metalworking fluids</atitle><jtitle>Annals of microbiology</jtitle><stitle>Ann Microbiol</stitle><date>2014</date><risdate>2014</risdate><volume>64</volume><issue>2</issue><spage>831</spage><epage>838</epage><pages>831-838</pages><issn>1590-4261</issn><eissn>1869-2044</eissn><abstract>Microbial contamination of metalworking fluids (MWFs) causes biofouling and degradation and is also associated with several health hazards. Development of an effective control method is therefore essential to reduce microbial loading in MWFs. The present study investigated the efficacy and rapidity of UV radiation as a means of disinfection of MWFs under laboratory conditions to determine parameters that could be used to design an in-line UV reactor for enclosed machines. High and low concentrations (10⁴–10⁷ CFU/mL) of three indicator bacteria, Pseudomonas fluorescens, P. oleovorans subsp. lubricantis and Mycobacterium chelonae, were evaluated both as pure cultures and in combinations. The target organisms were irradiated with a high intensity (192 μW/cm², 55 W) UV lamp for different exposure time under both static and mixed conditions. For these Pseudomonas species with high concentrations of cells under static conditions, only a 56 % reduction was observed within 10 min of exposure, whereas under mixed condition, a 99 % reduction was achieved within 2 min of exposure. In contrast only 74 % reduction was observed for M. chelonae. However, with low concentrations of cells under mixed conditions, a 99.99 % and 82 % reduction in viable count was observed for the Pseudomonas sp. and M. chelonae, respectively. Similar results were observed for mixed culture combinations. Based on these observations high intensity UV in combination with mixing could be successfully used as a means of disinfection of MWFs within a short exposure time and the parameters obtained from the study could be implemented to design a plug flow UV reactor.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s13213-013-0722-x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext_linktorsrc |
identifier | ISSN: 1590-4261 |
ispartof | Annals of microbiology, 2014, Vol.64 (2), p.831-838 |
issn | 1590-4261 1869-2044 |
language | eng |
recordid | cdi_proquest_journals_2404397648 |
source | Springer Nature OA Free Journals |
subjects | Applied Microbiology Biodegradation Biofouling Biomedical and Life Sciences Cell culture control methods Disinfection exposure duration health hazards indicator species Life Sciences lubricants Medical Microbiology Microbial contamination Microbial Ecology Microbial Genetics and Genomics Microbiology Mixed culture mixing Mycobacterium chelonae Mycology Original Article Pseudomonas Pseudomonas fluorescens reduction Ultraviolet radiation |
title | Determination of the effectiveness of UV radiation as a means of disinfection of metalworking fluids |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T19%3A21%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_C6C&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20the%20effectiveness%20of%20UV%20radiation%20as%20a%20means%20of%20disinfection%20of%20metalworking%20fluids&rft.jtitle=Annals%20of%20microbiology&rft.au=Saha,%20Ratul&rft.date=2014&rft.volume=64&rft.issue=2&rft.spage=831&rft.epage=838&rft.pages=831-838&rft.issn=1590-4261&rft.eissn=1869-2044&rft_id=info:doi/10.1007/s13213-013-0722-x&rft_dat=%3Cproquest_C6C%3E2404397648%3C/proquest_C6C%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2404397648&rft_id=info:pmid/&rfr_iscdi=true |