Transposable elements in chromosome-nuclear envelope attachment sites in nurse cells of malaria mosquitoes

A study is made of the DNA sequence at the attachment sites for XL chromosome of ovarian nurse cells in Anopheles messeae malarial mosquitos. This DNA sequence is isolated by the microdissection technique and is used for the creation of a clone library and for sequencing. The following transposable elements are found at the attachment site: LINE retrotransposons, LTR retrotransposons, and transposons. A search for these transposable elements is performed at the attachment sites of 3R chromosome of An. messeae and XL chromosome of An. atroparvus , as well as in the pericentric heterochromatin of chromosome 2 of, which is not connected to the nuclear envelope. The chromosome attachment sites studied have different transposable element sets. Some DNA clones contain transposable elements, SAR/MAR, and/or nuclear lamina DNA. It is suggested that the formation of the site for the attachment of XL chromosome of An. messeae might be caused by TE-mediated transposition of specific DNA sequences connected to the nuclear envelope.