Stability Enhancement of Silver Nanoparticles Through Surface Encapsulation via a Facile Green Synthesis Approach and Toxicity Reduction

Persuaded by the necessity of reduction of toxicity, silver nanoparticles (Ag-NPs) were synthesized from the reaction between AgNO 3 solution and Mizuna ( Brassica rapa var . japonica ) leaf extract. This study aimed to investigate the role of synthesis temperature on particle properties. Thus, four synthesis-temperatures, 25 °C (room temperature), 60 °C, 80 °C, and 100 °C were applied. Ag-NPs were characterized using ultraviolet–visible (UV–Vis) spectrophotometer, energy dispersive X-ray spectrometer, X-ray diffractometer, transmission electron microscope, atomic absorption spectrometry and dynamic light scattering techniques. The high-temperature (80 and 100 °C) synthesis showed higher stability, demostrated lowest release of silver ion (0.02 µg/mL, 0.002 µg/mL) compared to low-temperature synthesis. In addition, high-temperature synthesis showed higher C-content (> 50%) with lower Ag-content (