EFFECTIVE PLANT REGENERATION METHOD FROM CALLUS CULTURES, OBTAINED FROM DIFFERENT EMBRYO SOURCES, IN WINTER WHEAT (TRITICUM AESTIVUM L.) WITH THIDIAZURON (TDZ)

The different embryonic resources of certain winter wheat cultivars (Triticum aestivum L.) are explained in vitro in order to improve an effective plant regeneration system through callus inititiation, using different thidiazuron (TDZ) doses. In the case of each genotype or embryo source, thidiazuron has been seen to benefit plant regeneration by increasing the number of plants regenerated and transferred into the soil at a rate of between 1.18 and 3.7 times the controls. Within the embryo sources, immature embryos and within the cultivars, Bezostaja-1 responded to TDZ based common winter wheat regeneration treatments by producing the highest number of regenerants. Nevertheless, the 1 mg l-1 dose of TDZ caused significant reductions within the studied parameters. The method applied in this research can be used in winter wheat, gene transfer studies and transgenic plant production.