4CPS-196 Stability of lipid emulsion in paediatric parenteral nutrition with high electrolytic load

Background and importanceTernary mixtures in parenteral nutrition (PN) have a complex composition and so interactions between components can lead to instability, compromising safety. Fat globules >5 µm can cause thromboembolisms. Critical aggregation number (CAN) is used to predict stability (calculated with cation concentration).Aim and objectivesTo analyse the stability of the lipid emulsion in PN samples with a high CAN using globule size measurements, and to evaluate the influence of temperature and time on emulsion stability.Material and methodsWe studied four samples according to the nutritional requirements of a 1 kg neonate during the first days of life. Micronutrient amounts were greater than those recommended, and vitamins and zinc were also added. Samples were prepared in duplicate.Globule size was measured by laser diffraction (Beckman Coulter LS-I3-320) on the preparation day (day 0) and after 7 days. The samples were stored under refrigerated conditions and at room temperature. CAN was calculated based on the concentrations of cations present in each PN. Statistical analysis was performed using the Student’s t test (statistical significance p