Effect of hydropriming on Trigonella foenum callus growth, biochemical traits and phytochemical components under PEG treatment
The induction of secondary metabolites under osmotic stress is well documented. However, cell death is probably due to osmotic stress. This work tries to study the synergetic effect of hydropriming and polyethylene glycol (PEG) on enhancing the secondary metabolites production in fenugreek callus cu...
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| description | The induction of secondary metabolites under osmotic stress is well documented. However, cell death is probably due to osmotic stress. This work tries to study the synergetic effect of hydropriming and polyethylene glycol (PEG) on enhancing the secondary metabolites production in fenugreek callus cultures without facing cell death. PEG initiates the stress and the hydropriming increase the plant cell response against the stress. Fenugreek calli were initiated from hypocotyl of two groups of seeds, the first was hydroprimed overnight before germination, the second remained dry. Three months old calli of the two groups were subcultured on media containing two different concentration of PEG (5, 10%). The calli growth, biochemical analysis, secondary metabolism keys, and secondary metabolites were determined after 4 weeks. PE induced oxidative stress, which increased the membrane lipid peroxidation and decreased cell viability and growth. Hydropriming enhanced the activity of antioxidant enzymes, regulating the reactive oxygen species level, accumulating the osmolytes and secondary products. Therefore the primed callus can tolerate the osmotic stress initiated with PEG. Consequently, cell biomass increased and not affected by PEG treatment. On the other hand, the calli from non-primed seeds have a significant decrease in fresh weight, and dry weight under the higher PEG treatment. The hydropriming protected the growth of the cells under PEG treatment with a high content of secondary metabolites and high antioxidant machinery. The synergetic effect of hydropriming and PEG can be used as a simple and low-cost way to produce valuable compounds in commercial industrial bioreactors.
Key message
The synergetic effect of hydropriming and PEG enhances the secondary metabolites production in fenugreek callus. PEG initiates the stress and the hydropriming improves the plant cell response against the stress. |
| doi_str_mv | 10.1007/s11240-020-01778-6 |
| format | Article |
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Key message
The synergetic effect of hydropriming and PEG enhances the secondary metabolites production in fenugreek callus. PEG initiates the stress and the hydropriming improves the plant cell response against the stress.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-020-01778-6</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antioxidants ; Apoptosis ; Biochemical analysis ; Biomedical and Life Sciences ; Bioreactors ; Callus ; Cell death ; Cell viability ; Fenugreek ; Germination ; Life Sciences ; Lipid peroxidation ; Lipids ; Metabolites ; Original Article ; Osmotic stress ; Oxidative stress ; Peroxidation ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Polyethylene glycol ; Reactive oxygen species ; Secondary metabolites ; Seeds ; Trigonella foenum-graecum ; Weight</subject><ispartof>Plant cell, tissue and organ culture, 2020-04, Vol.141 (1), p.179-190</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Plant Cell, Tissue and Organ Culture (PCTOC) is a copyright of Springer, (2020). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-c7195b55efa7b5ff2b825e09386d474fe930777186b68cbcdf4290913b78466a3</citedby><cites>FETCH-LOGICAL-c356t-c7195b55efa7b5ff2b825e09386d474fe930777186b68cbcdf4290913b78466a3</cites><orcidid>0000-0001-7164-7838</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-020-01778-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-020-01778-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Alzandi, Abdulrahman Ali</creatorcontrib><creatorcontrib>Naguib, Deyala M.</creatorcontrib><title>Effect of hydropriming on Trigonella foenum callus growth, biochemical traits and phytochemical components under PEG treatment</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>The induction of secondary metabolites under osmotic stress is well documented. However, cell death is probably due to osmotic stress. This work tries to study the synergetic effect of hydropriming and polyethylene glycol (PEG) on enhancing the secondary metabolites production in fenugreek callus cultures without facing cell death. PEG initiates the stress and the hydropriming increase the plant cell response against the stress. Fenugreek calli were initiated from hypocotyl of two groups of seeds, the first was hydroprimed overnight before germination, the second remained dry. Three months old calli of the two groups were subcultured on media containing two different concentration of PEG (5, 10%). The calli growth, biochemical analysis, secondary metabolism keys, and secondary metabolites were determined after 4 weeks. PE induced oxidative stress, which increased the membrane lipid peroxidation and decreased cell viability and growth. Hydropriming enhanced the activity of antioxidant enzymes, regulating the reactive oxygen species level, accumulating the osmolytes and secondary products. Therefore the primed callus can tolerate the osmotic stress initiated with PEG. Consequently, cell biomass increased and not affected by PEG treatment. On the other hand, the calli from non-primed seeds have a significant decrease in fresh weight, and dry weight under the higher PEG treatment. The hydropriming protected the growth of the cells under PEG treatment with a high content of secondary metabolites and high antioxidant machinery. The synergetic effect of hydropriming and PEG can be used as a simple and low-cost way to produce valuable compounds in commercial industrial bioreactors.
Key message
The synergetic effect of hydropriming and PEG enhances the secondary metabolites production in fenugreek callus. PEG initiates the stress and the hydropriming improves the plant cell response against the stress.</description><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Biochemical analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Callus</subject><subject>Cell death</subject><subject>Cell viability</subject><subject>Fenugreek</subject><subject>Germination</subject><subject>Life Sciences</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Metabolites</subject><subject>Original Article</subject><subject>Osmotic stress</subject><subject>Oxidative stress</subject><subject>Peroxidation</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Polyethylene glycol</subject><subject>Reactive oxygen species</subject><subject>Secondary metabolites</subject><subject>Seeds</subject><subject>Trigonella foenum-graecum</subject><subject>Weight</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kM9LwzAUx4MoOKf_gKeAV6tJ0yTtUcacwkAP8xzSNGk72qQmLbKLf7uZFXbz8Hjwvj8efAC4xegBI8QfA8ZphhKUxsGc5wk7AwtMOUkoyrJzsECY8YTllF-CqxD2CCFGMrwA32tjtBqhM7A5VN4Nvu1bW0Nn4c63tbO66yQ0Ttuph0p23RRg7d3X2NzDsnWq0X0bz3D0sh0DlLaCQ3MYT4Jy_RBbbBQnW2kP39eb6NZy7OPxGlwY2QV987eX4ON5vVu9JNu3zevqaZsoQtmYKI4LWlKqjeQlNSYt85RqVJCcVRnPjC4I4pzjnJUsV6WqTJYWqMCk5HnGmCRLcDf3Dt59TjqMYu8mb-NLkZI8ljOK0uhKZ5fyLgSvjTjikP4gMBJHzmLmLCJn8ctZsBgicyhEs621P1X_k_oBaXOCOw</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Alzandi, Abdulrahman Ali</creator><creator>Naguib, Deyala M.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><orcidid>https://orcid.org/0000-0001-7164-7838</orcidid></search><sort><creationdate>20200401</creationdate><title>Effect of hydropriming on Trigonella foenum callus growth, biochemical traits and phytochemical components under PEG treatment</title><author>Alzandi, Abdulrahman Ali ; Naguib, Deyala M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-c7195b55efa7b5ff2b825e09386d474fe930777186b68cbcdf4290913b78466a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Biochemical analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Callus</topic><topic>Cell death</topic><topic>Cell viability</topic><topic>Fenugreek</topic><topic>Germination</topic><topic>Life Sciences</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Metabolites</topic><topic>Original Article</topic><topic>Osmotic stress</topic><topic>Oxidative stress</topic><topic>Peroxidation</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Polyethylene glycol</topic><topic>Reactive oxygen species</topic><topic>Secondary metabolites</topic><topic>Seeds</topic><topic>Trigonella foenum-graecum</topic><topic>Weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alzandi, Abdulrahman Ali</creatorcontrib><creatorcontrib>Naguib, Deyala M.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alzandi, Abdulrahman Ali</au><au>Naguib, Deyala M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of hydropriming on Trigonella foenum callus growth, biochemical traits and phytochemical components under PEG treatment</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2020-04-01</date><risdate>2020</risdate><volume>141</volume><issue>1</issue><spage>179</spage><epage>190</epage><pages>179-190</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>The induction of secondary metabolites under osmotic stress is well documented. However, cell death is probably due to osmotic stress. This work tries to study the synergetic effect of hydropriming and polyethylene glycol (PEG) on enhancing the secondary metabolites production in fenugreek callus cultures without facing cell death. PEG initiates the stress and the hydropriming increase the plant cell response against the stress. Fenugreek calli were initiated from hypocotyl of two groups of seeds, the first was hydroprimed overnight before germination, the second remained dry. Three months old calli of the two groups were subcultured on media containing two different concentration of PEG (5, 10%). The calli growth, biochemical analysis, secondary metabolism keys, and secondary metabolites were determined after 4 weeks. PE induced oxidative stress, which increased the membrane lipid peroxidation and decreased cell viability and growth. Hydropriming enhanced the activity of antioxidant enzymes, regulating the reactive oxygen species level, accumulating the osmolytes and secondary products. Therefore the primed callus can tolerate the osmotic stress initiated with PEG. Consequently, cell biomass increased and not affected by PEG treatment. On the other hand, the calli from non-primed seeds have a significant decrease in fresh weight, and dry weight under the higher PEG treatment. The hydropriming protected the growth of the cells under PEG treatment with a high content of secondary metabolites and high antioxidant machinery. The synergetic effect of hydropriming and PEG can be used as a simple and low-cost way to produce valuable compounds in commercial industrial bioreactors.
Key message
The synergetic effect of hydropriming and PEG enhances the secondary metabolites production in fenugreek callus. PEG initiates the stress and the hydropriming improves the plant cell response against the stress.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-020-01778-6</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-7164-7838</orcidid></addata></record> |
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| subjects | Antioxidants Apoptosis Biochemical analysis Biomedical and Life Sciences Bioreactors Callus Cell death Cell viability Fenugreek Germination Life Sciences Lipid peroxidation Lipids Metabolites Original Article Osmotic stress Oxidative stress Peroxidation Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Polyethylene glycol Reactive oxygen species Secondary metabolites Seeds Trigonella foenum-graecum Weight |
| title | Effect of hydropriming on Trigonella foenum callus growth, biochemical traits and phytochemical components under PEG treatment |
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