RBOH expression and ROS metabolism in Citrullus colocynthis under cadmium stress
NADPH oxidase (NOX) is a critical enzyme expressing by RBOH gene. NOX activity maintains a healthy level of reactive oxygen species (ROS) which are the natural by-product of oxygen metabolism. We aim to investigate the RBOH expression and the ROS metabolism in Citrullus colocynthis (L.) Schrad. plan...
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| Veröffentlicht in: | Brazilian Journal of Botany 2020-03, Vol.43 (1), p.35-43 |
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| creator | Khafi, Arezoo Sadat Iranbakhsh, Alireza Safipour Afshar, Akbar Khavari Nejad, Ramazan Ali |
| description | NADPH oxidase (NOX) is a critical enzyme expressing by RBOH gene. NOX activity maintains a healthy level of reactive oxygen species (ROS) which are the natural by-product of oxygen metabolism. We aim to investigate the RBOH expression and the ROS metabolism in
Citrullus colocynthis
(L.) Schrad. plant under cadmium (Cd) stress. Changes in diverse physiological and molecular characteristics were monitored following different treatments of CdCl
2
(0, 100, 200 and 300 μM). Results showed that shoot and root dry weight and length, plus relative water content, reduced significantly in comparison with control after Cd treatment. On the other hand, Cd exposure caused over-accumulation of H
2
O
2
and proline, whereas the Cd treatments led to inductions in the activities of catalase and superoxide dismutase enzyme. In addition, Cd exposure enhanced lipid peroxidation level, indicating oxidative damage to the membrane. Real-time PCR assay revealed that Cd up-regulated the expression of RBOH. |
| doi_str_mv | 10.1007/s40415-020-00581-z |
| format | Article |
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Citrullus colocynthis
(L.) Schrad. plant under cadmium (Cd) stress. Changes in diverse physiological and molecular characteristics were monitored following different treatments of CdCl
2
(0, 100, 200 and 300 μM). Results showed that shoot and root dry weight and length, plus relative water content, reduced significantly in comparison with control after Cd treatment. On the other hand, Cd exposure caused over-accumulation of H
2
O
2
and proline, whereas the Cd treatments led to inductions in the activities of catalase and superoxide dismutase enzyme. In addition, Cd exposure enhanced lipid peroxidation level, indicating oxidative damage to the membrane. Real-time PCR assay revealed that Cd up-regulated the expression of RBOH.</description><identifier>ISSN: 0100-8404</identifier><identifier>EISSN: 1806-9959</identifier><identifier>DOI: 10.1007/s40415-020-00581-z</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Biomedical and Life Sciences ; Botany ; Cadmium ; Cadmium chloride ; Catalase ; Citrullus colocynthis ; Enzymes ; Ethylenediaminetetraacetic acid ; Hydrogen peroxide ; Life Sciences ; Lipid peroxidation ; Lipids ; Metabolism ; Moisture content ; NAD(P)H oxidase ; Original Article ; Oxidases ; Oxygen metabolism ; Peroxidation ; Physiological aspects ; Plant Systematics/Taxonomy/Biogeography ; Povidone ; Proline ; Reactive oxygen species ; Superoxide ; Superoxide dismutase ; Water content</subject><ispartof>Brazilian Journal of Botany, 2020-03, Vol.43 (1), p.35-43</ispartof><rights>Botanical Society of Sao Paulo 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>2020© Botanical Society of Sao Paulo 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-49a1c5b84deadcfe8327e43455cbed27eb3825f89e031404ea591474178f6d023</citedby><cites>FETCH-LOGICAL-c420t-49a1c5b84deadcfe8327e43455cbed27eb3825f89e031404ea591474178f6d023</cites><orcidid>0000-0001-5879-0613</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40415-020-00581-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40415-020-00581-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Khafi, Arezoo Sadat</creatorcontrib><creatorcontrib>Iranbakhsh, Alireza</creatorcontrib><creatorcontrib>Safipour Afshar, Akbar</creatorcontrib><creatorcontrib>Khavari Nejad, Ramazan Ali</creatorcontrib><title>RBOH expression and ROS metabolism in Citrullus colocynthis under cadmium stress</title><title>Brazilian Journal of Botany</title><addtitle>Braz. J. Bot</addtitle><description>NADPH oxidase (NOX) is a critical enzyme expressing by RBOH gene. NOX activity maintains a healthy level of reactive oxygen species (ROS) which are the natural by-product of oxygen metabolism. We aim to investigate the RBOH expression and the ROS metabolism in
Citrullus colocynthis
(L.) Schrad. plant under cadmium (Cd) stress. Changes in diverse physiological and molecular characteristics were monitored following different treatments of CdCl
2
(0, 100, 200 and 300 μM). Results showed that shoot and root dry weight and length, plus relative water content, reduced significantly in comparison with control after Cd treatment. On the other hand, Cd exposure caused over-accumulation of H
2
O
2
and proline, whereas the Cd treatments led to inductions in the activities of catalase and superoxide dismutase enzyme. In addition, Cd exposure enhanced lipid peroxidation level, indicating oxidative damage to the membrane. Real-time PCR assay revealed that Cd up-regulated the expression of RBOH.</description><subject>Biomedical and Life Sciences</subject><subject>Botany</subject><subject>Cadmium</subject><subject>Cadmium chloride</subject><subject>Catalase</subject><subject>Citrullus colocynthis</subject><subject>Enzymes</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Hydrogen peroxide</subject><subject>Life Sciences</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Metabolism</subject><subject>Moisture content</subject><subject>NAD(P)H oxidase</subject><subject>Original Article</subject><subject>Oxidases</subject><subject>Oxygen metabolism</subject><subject>Peroxidation</subject><subject>Physiological aspects</subject><subject>Plant Systematics/Taxonomy/Biogeography</subject><subject>Povidone</subject><subject>Proline</subject><subject>Reactive oxygen species</subject><subject>Superoxide</subject><subject>Superoxide dismutase</subject><subject>Water content</subject><issn>0100-8404</issn><issn>1806-9959</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouH78AU8Bz10nbbpJj-uirrCw4sc5ZNOpRtpkTVpw_fVmreBNckgYnmdm8hJywWDKAMRV5MBZmUEOGUApWfZ1QCZMwiyrqrI6JBNIWCYTdUxOYnwHyEUhqgl5eLxeLyl-bgPGaL2j2tX0cf1EO-z1xrc2dtQ6urB9GNp2iNT41pud699spIOrMVCj684OHY39vscZOWp0G_H89z4lL7c3z4tltlrf3S_mq8zwHPqMV5qZciN5jbo2DcoiF8gLXpZmg3V6bwqZl42sEAqW1kZdVowLzoRsZjXkxSm5HPtug_8YMPbq3Q_BpZEqTz-bCWCCJWo6Uq-6RWVd4_ugTTo1dtZ4h41N9bmAWRpXSZmEfBRM8DEGbNQ22E6HnWKg9lGrMWqVolY_UauvJBWjFBPsXjH87fKP9Q0svIFX</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Khafi, Arezoo Sadat</creator><creator>Iranbakhsh, Alireza</creator><creator>Safipour Afshar, Akbar</creator><creator>Khavari Nejad, Ramazan Ali</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><orcidid>https://orcid.org/0000-0001-5879-0613</orcidid></search><sort><creationdate>20200301</creationdate><title>RBOH expression and ROS metabolism in Citrullus colocynthis under cadmium stress</title><author>Khafi, Arezoo Sadat ; Iranbakhsh, Alireza ; Safipour Afshar, Akbar ; Khavari Nejad, Ramazan Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-49a1c5b84deadcfe8327e43455cbed27eb3825f89e031404ea591474178f6d023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomedical and Life Sciences</topic><topic>Botany</topic><topic>Cadmium</topic><topic>Cadmium chloride</topic><topic>Catalase</topic><topic>Citrullus colocynthis</topic><topic>Enzymes</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Hydrogen peroxide</topic><topic>Life Sciences</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Metabolism</topic><topic>Moisture content</topic><topic>NAD(P)H oxidase</topic><topic>Original Article</topic><topic>Oxidases</topic><topic>Oxygen metabolism</topic><topic>Peroxidation</topic><topic>Physiological aspects</topic><topic>Plant Systematics/Taxonomy/Biogeography</topic><topic>Povidone</topic><topic>Proline</topic><topic>Reactive oxygen species</topic><topic>Superoxide</topic><topic>Superoxide dismutase</topic><topic>Water content</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khafi, Arezoo Sadat</creatorcontrib><creatorcontrib>Iranbakhsh, Alireza</creatorcontrib><creatorcontrib>Safipour Afshar, Akbar</creatorcontrib><creatorcontrib>Khavari Nejad, Ramazan Ali</creatorcontrib><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><jtitle>Brazilian Journal of Botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khafi, Arezoo Sadat</au><au>Iranbakhsh, Alireza</au><au>Safipour Afshar, Akbar</au><au>Khavari Nejad, Ramazan Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RBOH expression and ROS metabolism in Citrullus colocynthis under cadmium stress</atitle><jtitle>Brazilian Journal of Botany</jtitle><stitle>Braz. J. Bot</stitle><date>2020-03-01</date><risdate>2020</risdate><volume>43</volume><issue>1</issue><spage>35</spage><epage>43</epage><pages>35-43</pages><issn>0100-8404</issn><eissn>1806-9959</eissn><abstract>NADPH oxidase (NOX) is a critical enzyme expressing by RBOH gene. NOX activity maintains a healthy level of reactive oxygen species (ROS) which are the natural by-product of oxygen metabolism. We aim to investigate the RBOH expression and the ROS metabolism in
Citrullus colocynthis
(L.) Schrad. plant under cadmium (Cd) stress. Changes in diverse physiological and molecular characteristics were monitored following different treatments of CdCl
2
(0, 100, 200 and 300 μM). Results showed that shoot and root dry weight and length, plus relative water content, reduced significantly in comparison with control after Cd treatment. On the other hand, Cd exposure caused over-accumulation of H
2
O
2
and proline, whereas the Cd treatments led to inductions in the activities of catalase and superoxide dismutase enzyme. In addition, Cd exposure enhanced lipid peroxidation level, indicating oxidative damage to the membrane. Real-time PCR assay revealed that Cd up-regulated the expression of RBOH.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40415-020-00581-z</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5879-0613</orcidid></addata></record> |
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| subjects | Biomedical and Life Sciences Botany Cadmium Cadmium chloride Catalase Citrullus colocynthis Enzymes Ethylenediaminetetraacetic acid Hydrogen peroxide Life Sciences Lipid peroxidation Lipids Metabolism Moisture content NAD(P)H oxidase Original Article Oxidases Oxygen metabolism Peroxidation Physiological aspects Plant Systematics/Taxonomy/Biogeography Povidone Proline Reactive oxygen species Superoxide Superoxide dismutase Water content |
| title | RBOH expression and ROS metabolism in Citrullus colocynthis under cadmium stress |
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