Characterization and Optimization of process parameter for maximum Poly (−β-) hydroxyl butyrate production by bacteria isolated from the soil

Poly (ß-hydroxybutyrate) (PHB) is a biodegradable bacterial plastic belongs to the family of polyhydroxyalkanoates (PHA), and it is produced in the stress conditions like excess carbon source and limitation of nitrogen sources. [...]the 15 times greater production cost of commercial PHB creates a major limitation when its compared to the petrochemical-based synthetic plastic material12. [...]the alternative strategies such as isolation of potent PHA producing bacteria, finding less expensive raw materials, optimization in fermentation conditions and easier downstream process are required to reduce the total production cost. Isolation and Screening of PHB Producing Bacteria: Soil samples were collected from the waste dumping area of SRM University, Kattankulathur, Tamil Nadu, India, for the isolation of PHB producing bacteria and were serially diluted by using sterile distilled water, followed by plating on LB agar supplemented with 10% D-Glucose. Effect of Temperature and pH on Biomass and PHB Production: The pH and temperature play a significant role in the synthesis of PHB and Biomass concentration, the isolate was incubated at various temperature ranges from 25°C to 40°C, and pH ranges from 4 to 9 for 24, and 48 hrs in 10% glucose supplemented 100ml of LB broth.