Lithium Carbonate as A Bluing Agent – A Comparative Study

The aim of this study is to assess the efficacy of 0.05% aqueous solution of Lithium Carbonate as an alternative to Ammonium hydroxide water as a bluing agent. After staining with Haematoxylin, the five test group slides were immersed in 0.05% aqueous solution of Lithium carbonate, while the five control group slides were immersed in ammonium hydroxide water for bluing. In this study, we aim to assess the efficacy of 0.05% aqueous solution of Lithium Carbonate as an alternative to Ammonium hydroxide water as a bluing agent. By comparing the graphs, it was evident that nuclear intensity, cytoplasmic intensity, bluing and clarity of details was better with 0.05% Lithium Carbonate when compared to Ammonium Hydroxide water (Fig.1-4).