Validated and Sensitive Liquid Chromatography-Spectrophotometric Method for the Estimation of Doxofylline in Bulk Drug Formulation

[...]the present work aim for the development of UV spectrophotometric and RPHPLC method and determination of Doxofylline with possible shortest retention time. According to the ICH guidelines,the proposed method has also been validated[13] MATERIALS AND METHODS: Methods: Instrumentation: Shimadzu (UV 1601) UV- visible spectrophotometer with quartz cells of 1cm optical length incorporated with a PC computer was used, HPLC instrument equipped with UV-detection chromatographic separation was achieved at ambient temperature on column symmetry C18 (4.6x250mm, 3.5pm). Literature review revealed that no UVSpectrophotometry and RP-HPLC method is reported for the estimation of Doxofylline in tablet dosage form. [...]it was thought worthwhile to develop simple, precise, accurate UV-Spectrophotometery and RPHPLC method for the estimation of Doxofylline in tablet dosage form.