Optimization of Fibrinolytic Enzyme from Streptomyces althioticus BN22

The results indicated that glucose (51,570 Eu/ml) as carbon source and yeast extract (54,625 Eu/ml) as nitrogen source showed the highest fibrinolytic enzyme activity among them. [...]this study showed its originality by focus, that marine Streptomyces althioticus is an important source for fibrinolytic enzyme production. Thrombin, Fibrinogen, Human Fibrin, Plasma, Glucose, Maltose, Sucrose, Fructose, Galactose, soluble starch, Ammonium chloride, Yeast Extract, Ammonium sulphate, Potassium nitrate, Urea, soya bean and Peptone was purchased from Himedia, Mumbai, India. 2 Sample Collection: The different marine soil samples were collected from Chennai coastal region namely Marina beach, Besantnagr beach and Neelankarai beach. 2.3 Screening of Fibrinolytic enzyme activity: 114 actinomycetes were isolated from the marine soil samples that were collected from chennai coastal region namely marina beach, besantnagr and neelankarai beach, were reported earlier[21]- Actinomycete strains were tested by fibrin plate method, plate containing fibrin 20.0 g/l, peptone 10.0g/l, sodium chloride 5.0g/l, agar 15.0g/l and pH7.0 and the plates were incubated at room temperature for 5-6 days[22] After incubation, all the fibrin agar plates were observed for the zone of clearance. The BLAST sequence helped in the construction of a neighbour tree and phylogenetic tree to assign the isolated actinomycetes to a particular species in taxonomical classification. [...]the sequence establishes the complete genetic identity of the organism.