Simultaneous Estimation and Validation of Canagliflozin and Metformin Hydrochloride in Bulk and Pharmaceutical Dosage Form by using RP-HPLC

Since the calories are lost from the body in the form of glucose in urine of the patients taking this drug, it causes a negative energy balance and loss of weight, which is again beneficial in patients of T2DM. [...]drugs which induce this enzyme e.g. rifampicin, phenytoin, ritonavir etc. will decrease the plasma level of Canagliflozin. [...]its dose has to be increased to 300 mg/day when used in combination with these drugs. Metformin decreases blood glucose levels by decreasing hepatic glucose production, decreasing intestinal absorption of glucose, and improving insulin sensitivity by increasing peripheral glucose uptake and utilization. Optimized chromatographic condition In the present study the separation of Metformine hydrochloride and Canagliflozin was achieved by using column Grace C18, (250·4.6mm,5p) with mobile phase consisting of mixture of methanol and KH2PO4 Buffer in the ratio of 90:10 at a flow rate 0.8 ml/min with uv detection wavelength of 222nm at ambient temperature.