In vitro - In vivo Cellular Reprogramming and Antioxidant potential of Herbal Drug: Fumaria officinalis

In enzymatic estimations assay, the Col-I, Ela-I and Hya-I of extract were assessed showing inhibitory concentration as Col-I (IC50:41.25), Ela-I (IC50:45.31) and Hya-I (IC50:30.55) respectively. [...]MeOH extract of Fumaria officinalis able to inhibit 50% of the activity of aging related enzymes Col-I, Ela-I and Hya-I. Skin aging is the natural process due to photo aging by environmental factors such as chronic UV radiation. The repetitive exposure to UV radiation cause accelerated physical changes in the skin and connective tissue through the formation of lipid peroxides, the cell contents and Reactive Oxygen Species (ROS)[1]. Hyaluronidase Inhibitory Activity Assay: A mix of 25pL of various level of samples (20-100pg/ mL) of plant extracts were prepared and 3 pL hyaluronidase from bovine serum albumin[Sigma A4503] was pre-incubated for 10 min at 37°C and then added 12pL phosphate buffer (300mM, pH 5.35) for 10 min at 37°C. Afterward, 10pL hyaluronic acid substrate [Sigma H5388, USA] was added and incubated for 45 min at 37°C. Decomposition reaction of hyaluronic acid was stopped by adding 100pL acidic albumin acids.