Simultaneous electrochemical determination of adenine and guanine using poly 2-naphthol orange film–modified electrode

A poly 2-naphthol orange (P2NO) has been employed for the simultaneous electrochemical detection of adenine (A) and guanine (G). The P2NO-modified electrode was fabricated by electrochemical polymerization using cyclic voltammetry by applying potentials between − 0.6 and 0.8 V at a scan rate of 50 mV/s in 0.1 M phosphate buffer (pH − 7.0) containing 1 mM naphthol orange. The formation of P2NO film on the electrode surface was confirmed by cyclic voltammetry and square wave voltammetry techniques. The modified P2NO electrode showed good electrochemical behavior towards detection of adenine and guanine. The obtained linear range was from 1.6 to 30 μM with a detection limit of 0.05 μM based on S/N = 3. The P2NO-modified electrode response towards detection of adenine and guanine was quite consistent, and it has been utilized for real sample analysis with fish DNA sperm samples and the obtained results are satisfactory.