The effect of interval training intensity on protein levels of ATGL and Perilipin 5 in visceral adipose tissue of type 2 diabetic male rats

In adipose tissue, adipose triglyceride lipase (ATGL) and perilipin5 (PLIN5) play an important role in the regulation of lipolysis. However, little is known about the effect of interval training intensity on ATGL and PLIN5 in visceral adipose tissue of type 2 diabetic male rats. In this study, forty male Wistar rats were assigned into two groups as follows: healthy control (HC) (n=8) and T2D (n=32) groups which were fed with their corresponding diets for 10 weeks. After the induction of diabetes, CD animals and 8 rats from the D group were sacrificed (first experiment), and the remaining T2D animals were divided to moderate intensity interval training (MIIT), high intensity interval training (HIIT) and control (DC2) groups. Interval training (HIIT, MIIT) was applied for 12 weeks, 5 sessions per week. The MIIT protocol included 13 bouts of 4-minute activity with equivalent intensity of 60-65% vo2max and the HIIT protocol included 10 bouts of 4-minute activity with equivalent intensity of 85-90% vo2max with 2-minute active rest periods. The Western Blot method was used to measure PLIN5 and ATGL protein levels. ANOVA and Tukey's test were used for data analysis. The results indicated that type 2 diabetes resulted in a significant increase in the protein levels of PLIN5 (p05/0). When compared to the CD2, there was no significant difference between the two training groups in protein levels of PLIN5 and ATGL (p>0.05). Our finding indicated that interval training, independent of the exercise intensity, resulted in suppression of diabetes-induced PLIN5 which may be involved in the stimulation of visceral adipose tissue lipolysis.