Partial characterization of the X chromosome of Diuraphis noxia (Hemiptera: Aphididae)

We present the first sequencing results after separation of the X chromosome of Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae), the largest known X chromosome described to date, using flow cytometry. The X chromosome of D. noxia is 0.1824 pg (1C) and an estimated 178.4 Mb (1C) in size. Mapping c...

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Veröffentlicht in:Entomological science 2020-03, Vol.23 (1), p.86-94
Hauptverfasser: Steyn, Louis, Bierman, Anandi, Burger, N. Francois V., Botha, Anna‐Maria
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Sprache:eng
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Zusammenfassung:We present the first sequencing results after separation of the X chromosome of Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae), the largest known X chromosome described to date, using flow cytometry. The X chromosome of D. noxia is 0.1824 pg (1C) and an estimated 178.4 Mb (1C) in size. Mapping confirmed that the X chromosome contains 13,799 protein coding genes, but with a slight bias towards GC richness when compared to the complete D. noxia genome. Here we present the first sequencing results after separation of the X chromosome of Diuraphis noxia (Kurdjumov, Hemiptera: Aphididae), the largest known X chromosomes described to date, using flow cytometry. The X chromosome of D. noxia is 0.18 pg (1C) and an estimated 178 Mb (1C) in size. Mapping confirmed that the X chromosome contains 13,799 protein coding genes, but with a slight bias towards GC richness when compared to the complete D. noxia genome.
ISSN:1343-8786
1479-8298
DOI:10.1111/ens.12401