Preparation of bovine hemoglobin surface molecularly imprinted cotton for selective protein recognition

[Display omitted] •Synthesize the MIC via surface imprinting technique.•The MIC based on degreasing cotton is environmental and economical.•The MIC has good stability and acceptable reusabilitye. We have developed a bovine hemoglobin (BHb) surface molecularly imprinted cotton based on degreasing cotton via surface imprinting technique for the efficient selective adsorption of BHb. The morphological structure of the samples was characterized by Scanning Electron Microscopy (SEM), and the chemical modification steps were characterized by Fourier Transform Infrared Spectroscopy (FTIR). The maximum adsorption capacity of the molecularly imprinted cotton (MIC) and non-imprinted cotton (NIC) for BHb was 62.95 mg/g and 8.32 mg/g, respectively, at the optimum pH value of 6.2. The kinetics studies demonstrated that the adsorption follows a pseudo-first-order kinetic model. The adsorption isotherm analysis indicated that the Langmuir adsorption isotherm fits well with the adsorption equilibrium data. Also, the selective adsorption shows the MIC has a good selectivity for BHb. In addition, the assessment of the reusability of the MIC was tested for five successive cycles revealed no significant decrease of the adsorption capacity. Electrophoretic analysis suggests the MIC were successfully applied to capture template proteins from the bovine blood sample.