Quantitative Determination of Tannic Acid in Quercus Species by High Performance Liquid Chromatography

Some phenolic acids (gallic, ellagic and syringic acids), ellagitannins (vescalagin, pterocarinin, castalagin, grandinin), sugars (starch), methyl oleanolate, methy betulate, condensed tannins (derivatives of catechin, gallocatechin and epicatechin ) and calcium oxalate have been found in some Quercus extracts besides tannic acid (as pentadigalloylglucose complex) (Evans, 2009; Tuttu, 2014,; Wiesneth & Jürgenliemk, 2017; Önal et al., 2005; Ikram & Nowshad, 1977; Nonaka et al., 1990; Zhang et al., 2015; Şöhretoğlu & Sakar, 2004). Phenolic acids (gallic, vanillic, syringic and ellagic acids) and flavon glycosides (quercetin, isorhamnetin and kaempferol with their glucosides, etc.), with hydrolyzable tannins have been extensively determined in different parts of the Quercus species (acorn, bark, heartwood, leaf, cork, husk of root or root) besides their nutgalls by HPLC, LC-MS (HPLC-ESI-MS, LC-MS/MS) or NMR (FT-NMR, 1H-NMR, 13C-NMR, etc) methods (Önal et al., 2005; Nonaka et al., 1990; Zhang et al., 2015; Garcia-Villalba et al., 2017; Karaoğul et al., 2016; EMA, 2010). [...]investigation of four commercial tannic acids prepared from gallnuts of Q. infectaria, pods of Caesalpinia spinasa, leaves of Rhus catynus ar R. cariaria and gallnuts of R. semialata by Normal and Reversed Phase HPLC analysis. [...]no record has been found in extracts of Quercus infectaria subsp. baissieri and Q.infectaria subsp. infectaria growing in Turkey and their contents of tannic acid in literature by HPLC or LC-MS methods. [...]this study is aimed to evaluate the tannic acid contents in different extracts of Q.infectaria subsp. infectaria and Q.infectaria subsp. baissieri galls by HPLC analysis.