A functionalized dumbbell probe-based cascading exponential amplification DNA machine enables amplified probing of microRNAs

A functionalized dumbbell probe (FDP) based amplification method, termed as a cascading exponential amplification DNA machine (CEA-DNA machine), has been developed to autonomously accumulate single G-quadruplexes (SGQs) and twin-G-quadruplexes (TGQs) for robust fluorescence signal-on probing of miRN...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2020-02, Vol.56 (11), p.1681-1684
Hauptverfasser:	Wang, Jie, Li, Shuhui, Xu, Jianguo, Lu, Yusheng, Lin, Min, Wang, Chaihung, Zhang, Chen, Lin, Guoxing, Jia, Lee
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Benzothiazoles - chemistry Biosensing Techniques - methods Cell Line, Tumor Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA Probes - chemistry DNA Probes - genetics Fluorescence Fluorescent Dyes - chemistry G-Quadruplexes Humans Inverted Repeat Sequences Limit of Detection MicroRNAs MicroRNAs - blood MicroRNAs - genetics Nucleic Acid Amplification Techniques - methods Nucleic Acid Hybridization Spectrometry, Fluorescence - methods
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Beschreibung
Zusammenfassung:	A functionalized dumbbell probe (FDP) based amplification method, termed as a cascading exponential amplification DNA machine (CEA-DNA machine), has been developed to autonomously accumulate single G-quadruplexes (SGQs) and twin-G-quadruplexes (TGQs) for robust fluorescence signal-on probing of miRNA-21. A functionalized dumbbell probe-based cascading exponential amplification DNA machine has been developed for robust determination of miRNA-21.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c9cc08289a