Surface-Enhanced Raman Scattering-Based Immunoassay of Two Cytokines in Human Gingival Crevicular Fluid Of Periodontal Disease
This study combined Raman technology and nanotechnology to detect periodontal inflammatory factors. We synthesized silver nanoparticles and made modifications to their surface to produce Ag NP-antibody probes. A sandwich immunoassay was developed for the sensitive detection of TNF-α and IL-1β. SERS...
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| Veröffentlicht in: | Journal of applied spectroscopy 2020, Vol.86 (6), p.1077-1083 |
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| container_issue | 6 |
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| container_title | Journal of applied spectroscopy |
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| creator | Yang, H. Zhang, M. L. Yao, L. H. Zhou, M. Wang, Q. Chen, Yu Ding, Y. |
| description | This study combined Raman technology and nanotechnology to detect periodontal inflammatory factors. We synthesized silver nanoparticles and made modifications to their surface to produce Ag NP-antibody probes. A sandwich immunoassay was developed for the sensitive detection of TNF-α and IL-1β. SERS intensity showed a good linear relationship with TNF-α and IL-1β concentrations in our study. The results of detection of these biomarkers in GCF indicate excellent specificity, high sensitivity, and great reproducibility of this immunoassay. Our immunoassay based on the SERS technique would facilitate clinical diagnoses of periodontal diseases. |
| doi_str_mv | 10.1007/s10812-020-00943-1 |
| format | Article |
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Our immunoassay based on the SERS technique would facilitate clinical diagnoses of periodontal diseases.</description><identifier>ISSN: 0021-9037</identifier><identifier>EISSN: 1573-8647</identifier><identifier>DOI: 10.1007/s10812-020-00943-1</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical Chemistry ; Antibodies ; Atomic/Molecular Structure and Spectra ; Biomarkers ; Cytokines ; Immunoassay ; Medical research ; Medicine, Experimental ; Nanoparticles ; Nanotechnology ; Periodontal disease ; Physics ; Physics and Astronomy ; Povidone ; Raman spectra ; Silver ; Viral antibodies</subject><ispartof>Journal of applied spectroscopy, 2020, Vol.86 (6), p.1077-1083</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>2020© Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-e238542381543674e672b59ee6c5c9dd21d40afc94b3ccaee89822f4867231823</citedby><cites>FETCH-LOGICAL-c420t-e238542381543674e672b59ee6c5c9dd21d40afc94b3ccaee89822f4867231823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10812-020-00943-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10812-020-00943-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Yang, H.</creatorcontrib><creatorcontrib>Zhang, M. 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The results of detection of these biomarkers in GCF indicate excellent specificity, high sensitivity, and great reproducibility of this immunoassay. Our immunoassay based on the SERS technique would facilitate clinical diagnoses of periodontal diseases.</description><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Atomic/Molecular Structure and Spectra</subject><subject>Biomarkers</subject><subject>Cytokines</subject><subject>Immunoassay</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Periodontal disease</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><subject>Povidone</subject><subject>Raman spectra</subject><subject>Silver</subject><subject>Viral antibodies</subject><issn>0021-9037</issn><issn>1573-8647</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kV1rFDEUhgdRcK3-Aa8CXnmRms-ZzGVd-7FQqHTrdUgzJ2vqTLImmere-NvNukJZEAkkcPI853B4m-YtJaeUkO5DpkRRhgkjmJBecEyfNQsqO45VK7rnzYIQRnFPePeyeZXzA6mUYmTR_FrPyRkL-Dx8NcHCgG7NZAJaW1MKJB82-KPJtbyapjlEk7PZoejQ3Y-IlrsSv_kAGfmArua9dlkF_2hGtEzw6O08moQuxtkP6Mahz7VfHGIo9f-Tz1D7vm5eODNmePP3PWm-XJzfLa_w9c3lanl2ja1gpGBgXElRLyoFbzsBbcfuZQ_QWmn7YWB0EMQ424t7bq0BUHU75oSqHKeK8ZPm3aHvNsXvM-SiH-KcQh2pGZeE90Jx9URtzAjaBxdLMnby2eqzlnZMyV7KSp3-g6pngMnbGMD5Wj8S3h8JlSnws2zMnLNerW-PWXZgbYo5J3B6m_xk0k5TovdR60PUukat_0StaZX4QcrbfWKQnrb7j_Ub2TiphA</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Yang, H.</creator><creator>Zhang, M. 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L.</creatorcontrib><creatorcontrib>Yao, L. H.</creatorcontrib><creatorcontrib>Zhou, M.</creatorcontrib><creatorcontrib>Wang, Q.</creatorcontrib><creatorcontrib>Chen, Yu</creatorcontrib><creatorcontrib>Ding, Y.</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Science</collection><jtitle>Journal of applied spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, H.</au><au>Zhang, M. L.</au><au>Yao, L. H.</au><au>Zhou, M.</au><au>Wang, Q.</au><au>Chen, Yu</au><au>Ding, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surface-Enhanced Raman Scattering-Based Immunoassay of Two Cytokines in Human Gingival Crevicular Fluid Of Periodontal Disease</atitle><jtitle>Journal of applied spectroscopy</jtitle><stitle>J Appl Spectrosc</stitle><date>2020</date><risdate>2020</risdate><volume>86</volume><issue>6</issue><spage>1077</spage><epage>1083</epage><pages>1077-1083</pages><issn>0021-9037</issn><eissn>1573-8647</eissn><abstract>This study combined Raman technology and nanotechnology to detect periodontal inflammatory factors. We synthesized silver nanoparticles and made modifications to their surface to produce Ag NP-antibody probes. A sandwich immunoassay was developed for the sensitive detection of TNF-α and IL-1β. SERS intensity showed a good linear relationship with TNF-α and IL-1β concentrations in our study. 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| subjects | Analytical Chemistry Antibodies Atomic/Molecular Structure and Spectra Biomarkers Cytokines Immunoassay Medical research Medicine, Experimental Nanoparticles Nanotechnology Periodontal disease Physics Physics and Astronomy Povidone Raman spectra Silver Viral antibodies |
| title | Surface-Enhanced Raman Scattering-Based Immunoassay of Two Cytokines in Human Gingival Crevicular Fluid Of Periodontal Disease |
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