Surface-Enhanced Raman Scattering-Based Immunoassay of Two Cytokines in Human Gingival Crevicular Fluid Of Periodontal Disease

This study combined Raman technology and nanotechnology to detect periodontal inflammatory factors. We synthesized silver nanoparticles and made modifications to their surface to produce Ag NP-antibody probes. A sandwich immunoassay was developed for the sensitive detection of TNF-α and IL-1β. SERS intensity showed a good linear relationship with TNF-α and IL-1β concentrations in our study. The results of detection of these biomarkers in GCF indicate excellent specificity, high sensitivity, and great reproducibility of this immunoassay. Our immunoassay based on the SERS technique would facilitate clinical diagnoses of periodontal diseases.