Preliminary Exploration on Anti-Fibrosis Effect of Kaempferol in Mice with Schistosoma Japonicum Infection

This study is to explore the effectiveness and mechanism of kaempferol on treatment of hepatic fibrosis induced by schistosoma egg. Thirty-six healthy male balb/c mice were randomly divided into 6 groups, including negative group, positive group, and 4 different dosages of kaempferol treatment groups. Each mouse was infected with 20 schistosoma Cercariae japonicum, except the ones in the negative group. Four weeks later, every infected mouse was administrated with 500mg/kg/day praziquantel for 2 days, and all kaempferol groups were followed by a 4-week administration of kaempferol with 5, 10, 15 and 20mg/kg/day respectively, while both control groups were administrated with normal saline. AH the mice were sacrificed on the 59th day after infection. The liver tissues were taken for Masson staining to detect collagen and real-time quantitative PCR to detect the mRNA expression of IL-13, collagen 1 and MMP-2. As a result, Masson stain showed that the optical density of the interested region in the positive group was significantly higher than that in the negative group (P