Novel Data on the Taxonomic Diversity, Distribution, and Host Plants of Leafmining Moths of the Family Gracillariidae (Lepidoptera) in Siberia, Based on DNA Barcoding

The Gracillariidae fauna of Siberia, the region that occupies almost half of the territory of the Russian Federation, remains poorly studied. During a DNA barcoding study of Gracillariidae in Siberia, based on analysis of larvae and pupae collected from their leaf mines on woody plants, we identified 41 species. Three gracillariids were identified only to genus: Caloptilia sp. (host plant Prunus padus), Parornix sp. (Malus sp.), and Phyllonorycter sp. (Crataegus sp.), representing poorly studied or undescribed species. Six species are reported here for the first time for Siberia: Callisto insperatella (from Novosibirsk and Tomsk provinces), Caloptilia alnivorella (Buryatia), Phyllonorycter ermani (Irkutsk Province), Ph. lantanella (Novosibirsk Province), Ph. pumilae (Omsk and Irkutsk provinces), and Ph. viciae (Krasnoyarsk Territory). Parornix pfaffenzelleri , found in Khakassia, is reported as a species new to Russia. Other 15 gracillariid species previously known from Siberia were recorded in new administrative regions. The invasive lime leafminer Phyllonorycter issikii was first documented in Tomsk Province and Krasnoyarsk Territory in 2017 and 2018, respectively. Seven new gracillariid-host plant associations were found: Parectopa ononidis on Lupinaster pentaphyllus, Sauterina hofmanniella on Lathyrus gmelinii, Caloptilia stigmatella on Salix kochiana, Callisto insperatella on Prunus virginiana, Parornix scoticella on Amelanchier sp., Phyllonorycter ermani on Alnus alnobetula subsp. fruticosa , and Phyllonorycter viciae on Vicia unijuga. The Gracillariidae fauna of Siberia has 51% similarity with that of European Russia and only 38% similarity with that of the Russian Far East.