Effect of basal lamina of ovarian follicle on T- and L-type Ca2+ currents in differentiated granulosa cells

Patch clamp experiments were conducted to study the effects of basal lamina (basement membrane) of chicken ovarian follicle on membrane Ca2+ currents in differentiated chicken granulosa cells in a homologous system. The whole cell patch clamp technique was used to simultaneously monitor membrane capacitance (an indirect measure of total cell surface area) and currents flowing through voltage-dependent Ca2+ channels (using Ba2+ as the charge carrier).