Effects of salidroside on myocardial contractility in exhausted rats

Objective To investigate the effect of salidroside on myocardial contractility in exhausted rats for providing a new idea on protection of cardiac exhaustive injury. Methods Forty male Sprague-Dawley rats were randomly divided into 4 groups (n=10): control group, exhaustive group, salidroside A and B group. Thomas method was employed to establish the exhaustive animal model. Salidroside A and B groups were given salidroside solution 15mg/(kg·d) and 30mg/(kg·d) for 2 weeks, respectively, and the same volume of 0.9% NaCl was given to the exhaustive and control groups by intraperitoneal injection for 2 weeks. Serum creatine kinase (CK), creatine kinase isoenzyme (CK-MB) and troponin I (TnI) levels were detected by ELISA. Langendorff apparatus was used to separate the rats' ventricular myocytes. Rats' myocardial cell contractile function and calcium transient were detected with IonOptix cell contraction and calcium concentration synchronous measurement system. Laser confocal microscopy line scanning imaging was p