Morphological Changes and the Expression of Oxidoreductase Genes of Brassica napus in Deep Culture Media Containing PEG

The aim of this research was to investigate the expression of important oxidoreductase genes involved in drought stress tolerance in two Brassica napus cultivars. For this purpose, morphological traits and the expression of four oxidoreductase genes within 0, 3, 6, 12, 24, 48 and 72 h after culture in deep culture medium containing 0, 3, 6, 12 and 15% polyethylene glycol 6000 (PEG 6000) were investigated. Results revealed that in both sensitive and tolerant cultivars, there was a declining trend in not only the leaf weight and area but also the stem weight and length with increasing the PEG level. In both cultivars, changes in the expression of NDH were approximately the same at different PEG levels. The tolerant cultivar showed higher expression of FNR than the sensitive cultivar. The expression of GR1 and GPX1 genes was higher in Hyola 308 than SLM 046.