Determining transaminase activity in bacterial libraries by time-lapse imaging
Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic paramete...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2019-11, Vol.55 (9), p.13538-13541 |
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| container_issue | 9 |
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| container_title | Chemical communications (Cambridge, England) |
| container_volume | 55 |
| creator | Rodrigues, Carlos J. C Sanches, João M de Carvalho, Carla C. C. R |
| description | Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic parameters, and the assessment of the effect of the substrate concentration on activity.
Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. The correlation between substrate concentration and luminance allows the screening of biocatalysts and determination of kinetic parameters. |
| doi_str_mv | 10.1039/c9cc07507k |
| format | Article |
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Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. The correlation between substrate concentration and luminance allows the screening of biocatalysts and determination of kinetic parameters.</description><subject>Benzaldehyde</subject><subject>Correlation analysis</subject><subject>Image analysis</subject><subject>Parameter identification</subject><subject>Substrates</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpd0EtLxDAQB_AgCq6rF-9CwYsI1TyaR49Sn7joRcFbmWanS9a-TLrCfnuzriiYy2SGH0PyJ-SY0QtGRX5pc2upllS_75AJEypLZWbedjd3madaZHKfHISwpPEwaSbk6RpH9K3rXLdIRg9dgNhAwATs6D7duE5cl1SxQe-gSRpXefAOQ1Ktk9G1mDYwRO1aWMQVh2Svhibg0U-dktfbm5fiPp093z0UV7PUCpaNqWRoBTdcZqis4tlcy1oCiporbYTgaCgAoKn4XGtFhagMNQzyLE6ZypmYkrPt3sH3HysMY9m6YLFpoMN-FUouqJFMaW4iPf1Hl_3Kd_F1UTEuJVWaRnW-Vdb3IXisy8HHP_l1yWi5ibYs8qL4jvYx4pMt9sH-ur_oxRciu3TV</recordid><startdate>20191107</startdate><enddate>20191107</enddate><creator>Rodrigues, Carlos J. C</creator><creator>Sanches, João M</creator><creator>de Carvalho, Carla C. C. R</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9989-1397</orcidid><orcidid>https://orcid.org/0000-0001-9089-2740</orcidid><orcidid>https://orcid.org/0000-0002-8142-3835</orcidid></search><sort><creationdate>20191107</creationdate><title>Determining transaminase activity in bacterial libraries by time-lapse imaging</title><author>Rodrigues, Carlos J. C ; Sanches, João M ; de Carvalho, Carla C. C. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-51ec328254e6c624d75f5ae3f2678332e80aaae8b2d776033b8081a94aaa16913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Benzaldehyde</topic><topic>Correlation analysis</topic><topic>Image analysis</topic><topic>Parameter identification</topic><topic>Substrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodrigues, Carlos J. C</creatorcontrib><creatorcontrib>Sanches, João M</creatorcontrib><creatorcontrib>de Carvalho, Carla C. C. R</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodrigues, Carlos J. C</au><au>Sanches, João M</au><au>de Carvalho, Carla C. C. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determining transaminase activity in bacterial libraries by time-lapse imaging</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><date>2019-11-07</date><risdate>2019</risdate><volume>55</volume><issue>9</issue><spage>13538</spage><epage>13541</epage><pages>13538-13541</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic parameters, and the assessment of the effect of the substrate concentration on activity.
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| ispartof | Chemical communications (Cambridge, England), 2019-11, Vol.55 (9), p.13538-13541 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_proquest_journals_2312550670 |
| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Benzaldehyde Correlation analysis Image analysis Parameter identification Substrates |
| title | Determining transaminase activity in bacterial libraries by time-lapse imaging |
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