Determining transaminase activity in bacterial libraries by time-lapse imaging

Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic parameters, and the assessment of the effect of the substrate concentration on activity. Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. The correlation between substrate concentration and luminance allows the screening of biocatalysts and determination of kinetic parameters.