Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli

Abstract A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit). T...

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Veröffentlicht in:	FEMS microbiology letters 2000-03, Vol.184 (2), p.231-235
Hauptverfasser:	Beard, Steven J., Hashim, Rohani, Wu, Guanghui, Binet, Marie R.B., Hughes, Martin N., Poole, Robert K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacteriology Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - metabolism Chromosome Mapping DNA Transposable Elements Drug Resistance, Microbial - genetics E coli Escherichia coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Genetics Inorganic phosphate transport Ion Transport Microbiology Mutagenesis Mutagenesis, Insertional Mutation Phosphates Phosphates - metabolism pitA pitA gene Resistant mutant Transportation systems Zinc Zinc - metabolism Zinc - pharmacology Zinc sulfate Zinc transport
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Zusammenfassung:	Abstract A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit). The pitA mutant accumulated reduced amounts of zinc(II) when exposed to 0.5–2.0 mM ZnSO4 during growth in Luria–Bertani medium.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.2000.tb09019.x