Generation of Marburg virus-like particles by co-expression of glycoprotein and matrix protein
Marburg virus (MARV), the causative agent of a severe hemorrhagic fever, has a characteristic filamentous morphology. Here we report that co-expression of MARV glycoprotein and matrix protein (VP40) in mammalian cells leads to spontaneous budding of filamentous particles strikingly similar to wild-t...
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| Veröffentlicht in: | Pathogens and disease 2004-01, Vol.40 (1), p.27-31 |
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| container_title | Pathogens and disease |
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| creator | Swenson, Dana L Warfield, Kelly L Kuehl, Kathleen Larsen, Thomas Hevey, Michael C Schmaljohn, Alan Bavari, Sina Aman, M Javad |
| description | Marburg virus (MARV), the causative agent of a severe hemorrhagic fever, has a characteristic filamentous morphology. Here we report that co-expression of MARV glycoprotein and matrix protein (VP40) in mammalian cells leads to spontaneous budding of filamentous particles strikingly similar to wild-type MARV. In addition, these particles elicit an immune response in BALB/c mice. The generation of non-replicating Marburg virus-like particles (VLPs) should significantly facilitate the research on molecular mechanisms of MARV assembly and release. Furthermore, VLPs may be an excellent vaccine candidate against Marburg infection. |
| doi_str_mv | 10.1016/S0928-82440300273-6 |
| format | Article |
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Here we report that co-expression of MARV glycoprotein and matrix protein (VP40) in mammalian cells leads to spontaneous budding of filamentous particles strikingly similar to wild-type MARV. In addition, these particles elicit an immune response in BALB/c mice. The generation of non-replicating Marburg virus-like particles (VLPs) should significantly facilitate the research on molecular mechanisms of MARV assembly and release. 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Furthermore, VLPs may be an excellent vaccine candidate against Marburg infection.</description><subject>Glycoproteins</subject><subject>Hemorrhage</subject><subject>Hemorrhagic fever</subject><subject>Immune response</subject><subject>Infectious diseases</subject><subject>Mammalian cells</subject><subject>Marburg disease</subject><subject>Marburgvirus</subject><subject>Matrix protein</subject><subject>Molecular modelling</subject><subject>Morphology</subject><subject>Physical characteristics</subject><subject>Proteins</subject><subject>Replication</subject><subject>Virus-like particles</subject><subject>Viruses</subject><subject>VP40 protein</subject><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqNi71uwjAUhS2kSiDKE7Bcidn02g4hmVF_lk5lYCIy4YIMwQ7XTgVv3wx5gJ7lk853jhBzhUuFKn_7wVIXstBZhgZRr43MR2KiMStlbvRuLGYxXrBPsVLFOp-I_Sd5Yptc8BBO8G350PEZfh13UTbuStBaTq5uKMLhCXWQ9GiZYhwO5-ZZh5ZDIufB-iPcbGL3gKF6FS8n20SaDZyKxcf7dvMle3_vKKbqEjr2vaq0wdKUuDLK_G_1B-MASqk</recordid><startdate>20040101</startdate><enddate>20040101</enddate><creator>Swenson, Dana L</creator><creator>Warfield, Kelly L</creator><creator>Kuehl, Kathleen</creator><creator>Larsen, Thomas</creator><creator>Hevey, Michael C</creator><creator>Schmaljohn, Alan</creator><creator>Bavari, Sina</creator><creator>Aman, M Javad</creator><general>Oxford University Press</general><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>20040101</creationdate><title>Generation of Marburg virus-like particles by co-expression of glycoprotein and matrix protein</title><author>Swenson, Dana L ; Warfield, Kelly L ; Kuehl, Kathleen ; Larsen, Thomas ; Hevey, Michael C ; Schmaljohn, Alan ; Bavari, Sina ; Aman, M Javad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_23093905313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Glycoproteins</topic><topic>Hemorrhage</topic><topic>Hemorrhagic fever</topic><topic>Immune response</topic><topic>Infectious diseases</topic><topic>Mammalian cells</topic><topic>Marburg disease</topic><topic>Marburgvirus</topic><topic>Matrix protein</topic><topic>Molecular modelling</topic><topic>Morphology</topic><topic>Physical characteristics</topic><topic>Proteins</topic><topic>Replication</topic><topic>Virus-like particles</topic><topic>Viruses</topic><topic>VP40 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swenson, Dana L</creatorcontrib><creatorcontrib>Warfield, Kelly L</creatorcontrib><creatorcontrib>Kuehl, Kathleen</creatorcontrib><creatorcontrib>Larsen, Thomas</creatorcontrib><creatorcontrib>Hevey, Michael C</creatorcontrib><creatorcontrib>Schmaljohn, Alan</creatorcontrib><creatorcontrib>Bavari, Sina</creatorcontrib><creatorcontrib>Aman, M Javad</creatorcontrib><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Pathogens and disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swenson, Dana L</au><au>Warfield, Kelly L</au><au>Kuehl, Kathleen</au><au>Larsen, Thomas</au><au>Hevey, Michael C</au><au>Schmaljohn, Alan</au><au>Bavari, Sina</au><au>Aman, M Javad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of Marburg virus-like particles by co-expression of glycoprotein and matrix protein</atitle><jtitle>Pathogens and disease</jtitle><date>2004-01-01</date><risdate>2004</risdate><volume>40</volume><issue>1</issue><spage>27</spage><epage>31</epage><pages>27-31</pages><eissn>2049-632X</eissn><abstract>Marburg virus (MARV), the causative agent of a severe hemorrhagic fever, has a characteristic filamentous morphology. 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| ispartof | Pathogens and disease, 2004-01, Vol.40 (1), p.27-31 |
| issn | 2049-632X |
| language | eng |
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| source | Access via Wiley Online Library |
| subjects | Glycoproteins Hemorrhage Hemorrhagic fever Immune response Infectious diseases Mammalian cells Marburg disease Marburgvirus Matrix protein Molecular modelling Morphology Physical characteristics Proteins Replication Virus-like particles Viruses VP40 protein |
| title | Generation of Marburg virus-like particles by co-expression of glycoprotein and matrix protein |
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