Generation of Marburg virus-like particles by co-expression of glycoprotein and matrix protein

Marburg virus (MARV), the causative agent of a severe hemorrhagic fever, has a characteristic filamentous morphology. Here we report that co-expression of MARV glycoprotein and matrix protein (VP40) in mammalian cells leads to spontaneous budding of filamentous particles strikingly similar to wild-t...

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Veröffentlicht in:Pathogens and disease 2004-01, Vol.40 (1), p.27-31
Hauptverfasser: Swenson, Dana L, Warfield, Kelly L, Kuehl, Kathleen, Larsen, Thomas, Hevey, Michael C, Schmaljohn, Alan, Bavari, Sina, Aman, M Javad
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Sprache:eng
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Zusammenfassung:Marburg virus (MARV), the causative agent of a severe hemorrhagic fever, has a characteristic filamentous morphology. Here we report that co-expression of MARV glycoprotein and matrix protein (VP40) in mammalian cells leads to spontaneous budding of filamentous particles strikingly similar to wild-type MARV. In addition, these particles elicit an immune response in BALB/c mice. The generation of non-replicating Marburg virus-like particles (VLPs) should significantly facilitate the research on molecular mechanisms of MARV assembly and release. Furthermore, VLPs may be an excellent vaccine candidate against Marburg infection.
ISSN:2049-632X
DOI:10.1016/S0928-82440300273-6