1H-NMR investigation of complex fonnation of ethidium bromide with single-stranded non-complementary deoxytetranucleotide 5'-d(CpGpApA) in aqueous solution

1H-NMR investigation of complex fonnation of ethidium bromide with single-stranded non-complementary deoxytetranucleotide 5'-d(CpGpApA) in aqueous solution

Complex formation between the aromatic dye ethidium bromide (3,8-diamino-6-phenyl-5-ethyl-phenan-thridine) and single-stranded non-complementary deoxytetranucleotide 5'-d(CpGpApA) in aqueous solution has been studied by one- and two-dimensional H-NMR spectroscopy (500 and 600 MHz). Concentratio...

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Veröffentlicht in:Biopolimery i kletka 1996-11, Vol.12 (6), p.36-49
Hauptverfasser: Veselkov, A. N., Baranovsky, S. F., Djimant, L. N., Petrenko, N. V., Osetrov, S. G., Tucker, A., Davies, D. B.
Format: Artikel
Sprache:eng
Schlagworte:
Aqueous solutions
Ethidium bromide
Magnetic resonance spectroscopy
NMR
Nuclear magnetic resonance
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Zusammenfassung:Complex formation between the aromatic dye ethidium bromide (3,8-diamino-6-phenyl-5-ethyl-phenan-thridine) and single-stranded non-complementary deoxytetranucleotide 5'-d(CpGpApA) in aqueous solution has been studied by one- and two-dimensional H-NMR spectroscopy (500 and 600 MHz). Concentration dependences of proton chemical shifts of the interacting molecules were measured at different temperatures T
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.000453