Generation of reactive oxygen species by neutrophils under their interaction with monocarboxycellulose

An influence of monocarboxycellulose (MCC) with different content of COOH-groups on the oxygen activation systems of neutrophils isolated from blood of healthy donors has been studied using tuminol dependent chemiluminescence (LCDL) method. The addition of MCC into cell suspension leads to the decrease in reactive oxygen species (ROS) yield at different stages of cells activation by adhesion to glass surface and latex particles. This effect does not depend on adhesivity of cells to MCC films. However, it depends on the concentration of carboxy-groups in MCC: the increase of percentage of COOH-groups in MCC from 0.26 % to 16 % causes a decrease in the ROS yield in neutrophils. MCC also causes a decrease in the myeloperoxidase activity in a model system «MPO– H