Investigation of some structural-functional peculiarities of the immunogenic fragment Ki-67 antigen. Generation of polyclonal and monoclonal antibodies to Ki-67
The study of tumor-associated antigens function is a prerequisite for understanding the molecular mechanisms of oncogenesis. Earlier we have determined the nuclear protein Ki-67 as an autoantigen in human melanoma. cDNA of immunogenic fragment of Ki-67 has been cloned into pET23d vector and expresse...
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Veröffentlicht in: | Biopolimery i kletka 2005, Vol.21 (2), p.180-183 |
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Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The study of tumor-associated antigens function is a prerequisite for understanding the molecular mechanisms of oncogenesis. Earlier we have determined the nuclear protein Ki-67 as an autoantigen in human melanoma. cDNA of immunogenic fragment of Ki-67 has been cloned into pET23d vector and expressed in bacterial cells. The recombinant fragment of Ki-67 has been purified from the bacterial cell lysate has been used as an antigen for the production of polyclonal and monoclonal antibodies with following detection of Ki-67 in Breast adenocarcinomas. We have shown by the immunodot-blot analysis that the 18 kDa recombinant fragment of Ki-67 possesses a DNA-binding activity. |
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ISSN: | 0233-7657 1993-6842 |
DOI: | 10.7124/bc.0006EA |