Peculiarities of antistress proteins Hsp60 and P450 2E1 expression at dilated cardiomyopathy
The data concerning peculiarities of abundant mitochondrial chaperon Hsp60 and main microsomal cytochrome P450 monooxigenase (2E1 isoform) expression at dilated cardiomyopathy (DCM) progression at the end stage of heart failure have been obtained using Western-blot analysis. The ischemic and normal...
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| Veröffentlicht in: | Biopolimery i kletka 2004-09, Vol.20 (5), p.429-434 |
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| creator | Sidorik, L. L. Bobyk, V. I. Kyyamova, R. G. Kapustjan, L. N. Rozhko, O. T. Vigontina, O. G. Ryabenko, D. V. Danko, I. M. Maksymchuk, O. V. Filonenko, V. V. Kovalenko, V. N. Chaschin, N. A. |
| description | The data concerning peculiarities of abundant mitochondrial chaperon Hsp60 and main microsomal cytochrome P450 monooxigenase (2E1 isoform) expression at dilated cardiomyopathy (DCM) progression at the end stage of heart failure have been obtained using Western-blot analysis. The ischemic and normal human hearts were studied as a control. We observed a decrease in Hsp60 level in cytoplasmic fraction of DCM- and ischemia-affected hearts’ left ventricular and significant increase in Hsp60 in mitochondrial fractions of all the hearts investigated. At the same time we detected an increase in P450 2E1 expression level in the ischemic and dilated hearts’ cytoplasmic fractions in comparison with the normal myocardium while no changes in microsomal fractions of the hearts investigated were detected. This could be related to the increased level of oxidative injury of DCM heart muscle. In addition, all changes described are accompanied by a significant decrease in the ATPase activity of myosin purified from the DCM-affected heart in comparison with the normal and ischemic myocardia as well as an increase in specific antimyocardial autoantibodies level in DCM patients sera. The working hypothesis concerning functional relationship between the antistress (HSPs) and antioxidative (cytochromes) systems at DCM progression is proposed. |
| doi_str_mv | 10.7124/bc.0006C6 |
| format | Article |
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L. ; Bobyk, V. I. ; Kyyamova, R. G. ; Kapustjan, L. N. ; Rozhko, O. T. ; Vigontina, O. G. ; Ryabenko, D. V. ; Danko, I. M. ; Maksymchuk, O. V. ; Filonenko, V. V. ; Kovalenko, V. N. ; Chaschin, N. A.</creator><creatorcontrib>Sidorik, L. L. ; Bobyk, V. I. ; Kyyamova, R. G. ; Kapustjan, L. N. ; Rozhko, O. T. ; Vigontina, O. G. ; Ryabenko, D. V. ; Danko, I. M. ; Maksymchuk, O. V. ; Filonenko, V. V. ; Kovalenko, V. N. ; Chaschin, N. A.</creatorcontrib><description>The data concerning peculiarities of abundant mitochondrial chaperon Hsp60 and main microsomal cytochrome P450 monooxigenase (2E1 isoform) expression at dilated cardiomyopathy (DCM) progression at the end stage of heart failure have been obtained using Western-blot analysis. The ischemic and normal human hearts were studied as a control. We observed a decrease in Hsp60 level in cytoplasmic fraction of DCM- and ischemia-affected hearts’ left ventricular and significant increase in Hsp60 in mitochondrial fractions of all the hearts investigated. At the same time we detected an increase in P450 2E1 expression level in the ischemic and dilated hearts’ cytoplasmic fractions in comparison with the normal myocardium while no changes in microsomal fractions of the hearts investigated were detected. This could be related to the increased level of oxidative injury of DCM heart muscle. In addition, all changes described are accompanied by a significant decrease in the ATPase activity of myosin purified from the DCM-affected heart in comparison with the normal and ischemic myocardia as well as an increase in specific antimyocardial autoantibodies level in DCM patients sera. The working hypothesis concerning functional relationship between the antistress (HSPs) and antioxidative (cytochromes) systems at DCM progression is proposed.</description><identifier>ISSN: 0233-7657</identifier><identifier>EISSN: 1993-6842</identifier><identifier>DOI: 10.7124/bc.0006C6</identifier><language>eng</language><publisher>Kiev: Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine</publisher><subject>Adenosine triphosphatase ; Autoantibodies ; Cardiac muscle ; Cardiomyopathy ; Congestive heart failure ; Cytochrome P450 ; Dilated cardiomyopathy ; Hsp60 protein ; Ischemia ; Mitochondria ; Myocardium ; Myosin ; Ventricle</subject><ispartof>Biopolimery i kletka, 2004-09, Vol.20 (5), p.429-434</ispartof><rights>2004. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c976-f163ae8665dace8f2c2f97a43aa6169536664e507663097dcf031fb3f80baa6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sidorik, L. L.</creatorcontrib><creatorcontrib>Bobyk, V. I.</creatorcontrib><creatorcontrib>Kyyamova, R. G.</creatorcontrib><creatorcontrib>Kapustjan, L. N.</creatorcontrib><creatorcontrib>Rozhko, O. T.</creatorcontrib><creatorcontrib>Vigontina, O. G.</creatorcontrib><creatorcontrib>Ryabenko, D. V.</creatorcontrib><creatorcontrib>Danko, I. M.</creatorcontrib><creatorcontrib>Maksymchuk, O. V.</creatorcontrib><creatorcontrib>Filonenko, V. V.</creatorcontrib><creatorcontrib>Kovalenko, V. N.</creatorcontrib><creatorcontrib>Chaschin, N. A.</creatorcontrib><title>Peculiarities of antistress proteins Hsp60 and P450 2E1 expression at dilated cardiomyopathy</title><title>Biopolimery i kletka</title><description>The data concerning peculiarities of abundant mitochondrial chaperon Hsp60 and main microsomal cytochrome P450 monooxigenase (2E1 isoform) expression at dilated cardiomyopathy (DCM) progression at the end stage of heart failure have been obtained using Western-blot analysis. The ischemic and normal human hearts were studied as a control. We observed a decrease in Hsp60 level in cytoplasmic fraction of DCM- and ischemia-affected hearts’ left ventricular and significant increase in Hsp60 in mitochondrial fractions of all the hearts investigated. At the same time we detected an increase in P450 2E1 expression level in the ischemic and dilated hearts’ cytoplasmic fractions in comparison with the normal myocardium while no changes in microsomal fractions of the hearts investigated were detected. This could be related to the increased level of oxidative injury of DCM heart muscle. In addition, all changes described are accompanied by a significant decrease in the ATPase activity of myosin purified from the DCM-affected heart in comparison with the normal and ischemic myocardia as well as an increase in specific antimyocardial autoantibodies level in DCM patients sera. The working hypothesis concerning functional relationship between the antistress (HSPs) and antioxidative (cytochromes) systems at DCM progression is proposed.</description><subject>Adenosine triphosphatase</subject><subject>Autoantibodies</subject><subject>Cardiac muscle</subject><subject>Cardiomyopathy</subject><subject>Congestive heart failure</subject><subject>Cytochrome P450</subject><subject>Dilated cardiomyopathy</subject><subject>Hsp60 protein</subject><subject>Ischemia</subject><subject>Mitochondria</subject><subject>Myocardium</subject><subject>Myosin</subject><subject>Ventricle</subject><issn>0233-7657</issn><issn>1993-6842</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNot0M9LwzAUB_AgCs7pwf8g4MlDZ360L-1RxnTCwB12FEKaJpjRNTVJwf73ZszTO7wP3_f4IvRIyUpQVr60ekUIgTVcoQVtGl5AXbJrtCCM80JAJW7RXYzHTMqasQX62hs99U4Fl5yJ2FushuRiCiZGPAafjBsi3sYRSN50eF9WBLMNxeZ3PBvnB6wS7lyvkumwVqFz_jT7UaXv-R7dWNVH8_A_l-jwtjmst8Xu8_1j_bordCOgsBS4MjVA1Sltass0s41QJVcKKDQVB4DSVEQAcNKITlvCqW25rUmbScuX6OkSm__9mUxM8uinMOSLknEiKK1yQFbPF6WDjzEYK8fgTirMkhJ57k62Wl664390H2Cw</recordid><startdate>20040920</startdate><enddate>20040920</enddate><creator>Sidorik, L. 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G.</creatorcontrib><creatorcontrib>Ryabenko, D. V.</creatorcontrib><creatorcontrib>Danko, I. M.</creatorcontrib><creatorcontrib>Maksymchuk, O. V.</creatorcontrib><creatorcontrib>Filonenko, V. V.</creatorcontrib><creatorcontrib>Kovalenko, V. N.</creatorcontrib><creatorcontrib>Chaschin, N. A.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Biopolimery i kletka</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sidorik, L. L.</au><au>Bobyk, V. I.</au><au>Kyyamova, R. G.</au><au>Kapustjan, L. N.</au><au>Rozhko, O. T.</au><au>Vigontina, O. G.</au><au>Ryabenko, D. V.</au><au>Danko, I. M.</au><au>Maksymchuk, O. V.</au><au>Filonenko, V. V.</au><au>Kovalenko, V. N.</au><au>Chaschin, N. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peculiarities of antistress proteins Hsp60 and P450 2E1 expression at dilated cardiomyopathy</atitle><jtitle>Biopolimery i kletka</jtitle><date>2004-09-20</date><risdate>2004</risdate><volume>20</volume><issue>5</issue><spage>429</spage><epage>434</epage><pages>429-434</pages><issn>0233-7657</issn><eissn>1993-6842</eissn><abstract>The data concerning peculiarities of abundant mitochondrial chaperon Hsp60 and main microsomal cytochrome P450 monooxigenase (2E1 isoform) expression at dilated cardiomyopathy (DCM) progression at the end stage of heart failure have been obtained using Western-blot analysis. The ischemic and normal human hearts were studied as a control. We observed a decrease in Hsp60 level in cytoplasmic fraction of DCM- and ischemia-affected hearts’ left ventricular and significant increase in Hsp60 in mitochondrial fractions of all the hearts investigated. 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| subjects | Adenosine triphosphatase Autoantibodies Cardiac muscle Cardiomyopathy Congestive heart failure Cytochrome P450 Dilated cardiomyopathy Hsp60 protein Ischemia Mitochondria Myocardium Myosin Ventricle |
| title | Peculiarities of antistress proteins Hsp60 and P450 2E1 expression at dilated cardiomyopathy |
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