Peculiarities of antistress proteins Hsp60 and P450 2E1 expression at dilated cardiomyopathy

The data concerning peculiarities of abundant mitochondrial chaperon Hsp60 and main microsomal cytochrome P450 monooxigenase (2E1 isoform) expression at dilated cardiomyopathy (DCM) progression at the end stage of heart failure have been obtained using Western-blot analysis. The ischemic and normal human hearts were studied as a control. We observed a decrease in Hsp60 level in cytoplasmic fraction of DCM- and ischemia-affected hearts’ left ventricular and significant increase in Hsp60 in mitochondrial fractions of all the hearts investigated. At the same time we detected an increase in P450 2E1 expression level in the ischemic and dilated hearts’ cytoplasmic fractions in comparison with the normal myocardium while no changes in microsomal fractions of the hearts investigated were detected. This could be related to the increased level of oxidative injury of DCM heart muscle. In addition, all changes described are accompanied by a significant decrease in the ATPase activity of myosin purified from the DCM-affected heart in comparison with the normal and ischemic myocardia as well as an increase in specific antimyocardial autoantibodies level in DCM patients sera. The working hypothesis concerning functional relationship between the antistress (HSPs) and antioxidative (cytochromes) systems at DCM progression is proposed.