Regulation of K-Cl cotransport during reticulocyte maturation and erythrocyte aging in normal and sickle erythrocytes

Regulation of K-Cl cotransport during reticulocyte maturation and erythrocyte aging in normal and sickle erythrocytes

The age/density-dependent decrease in K-Cl cotransport (KCC), PP1 and PP2A activities in normal and sickle human erythrocytes, and the effect of urea, a known KCC activator, were studied using discontinuous, isotonic gradients. In normal, least-dense but not in densest cells, urea stimulates KCC (tw...

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Veröffentlicht in:American Journal of Physiology: Cell Physiology 2003-07, Vol.54 (1), p.C31-C38
Hauptverfasser: BIZE, Isabel, TAHER, Samara, BRUGNARA, Carlo
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Cellular biology
Enzymes
Erythrocytes
Fundamental and applied biological sciences. Psychology
Proteins
Vertebrates: blood, hematopoietic organs, reticuloendothelial system
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Zusammenfassung:The age/density-dependent decrease in K-Cl cotransport (KCC), PP1 and PP2A activities in normal and sickle human erythrocytes, and the effect of urea, a known KCC activator, were studied using discontinuous, isotonic gradients. In normal, least-dense but not in densest cells, urea stimulates KCC (two- to fourfold) and moderately increases mb-PP1 (20%-40%). Thus mb-PP1 appears to mediate part of urea-stimulated KCC activity. [PUBLICATION ABSTRACT]
ISSN:0363-6143
1522-1563