Clathrin in mitotic spindles

Subconfluent cultures of Madin-Darby canine kidney (MDCK) and CV-1 cells were immunostained with two monoclonal antibodies (MAbs), MAb X-22 and MAb 23, against clathrin heavy chain and with polyclonal antiserum against a conserved region of all mammalian clathrin light chains. In interphase MDCK and...

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Veröffentlicht in:	American Journal of Physiology: Cell Physiology 2000-08, Vol.48 (2), p.369-374
Hauptverfasser:	OKAMOTO, C. T, MCKINNEY, J, JENG, Y. Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biological and medical sciences Cell cycle, cell proliferation Cell physiology Cellular biology Fundamental and applied biological sciences. Psychology Kidneys Molecular and cellular biology Pharmacology
Online-Zugang:	Volltext
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Zusammenfassung:	Subconfluent cultures of Madin-Darby canine kidney (MDCK) and CV-1 cells were immunostained with two monoclonal antibodies (MAbs), MAb X-22 and MAb 23, against clathrin heavy chain and with polyclonal antiserum against a conserved region of all mammalian clathrin light chains. In interphase MDCK and CV-1 cells, staining by all three antibodies resulted in the characteristic intracellular punctate vesicular and perinuclear staining pattern.
ISSN:	0363-6143 1522-1563