Evaluation of ovarian POMC mRNA through quantitative RT-PCR analysis in Rana esculenta

The evaluation of changes in the expression of specific genes requires accurate measurement of the corresponding mRNA concentration, especially when the gene is expressed at a very low level. We previously showed that hte proopiomelnocortin (POMC) gene is expressed in the ovary of the frog Rana esculenta, and, to to evaluate its mRNA concent in frog ovary, we have now developed a sensitive quantitative RT-PCR method.