Loading pyranine via purinergic receptors or hypotonic stress for measurement of cytosolic pH by imaging

Loading pyranine via purinergic receptors or hypotonic stress for measurement of cytosolic pH by imaging

Although used extensively for the measurement of intracellular pH, derivatives of fluorescein such as 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) have suboptimal sensitivity and can generate toxic photoproducts.

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Bibliographische Detailangaben
Veröffentlicht in:American Journal of Physiology: Cell Physiology 1998-10, Vol.44 (4), p.C1158-C1166
Hauptverfasser: BING SIANG GAN, KRUMP, E, SHRODE, L. D, GRINSTEIN, S
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Cellular biology
Diverse techniques
Fundamental and applied biological sciences. Psychology
Measurement
Molecular and cellular biology
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Beschreibung
Zusammenfassung:Although used extensively for the measurement of intracellular pH, derivatives of fluorescein such as 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) have suboptimal sensitivity and can generate toxic photoproducts.
ISSN:0363-6143
1522-1563