Characterization and imaging of A6 epithelial cell clones expressing fluorescently labeled ENaC subunits

A6 model renal epithelial cells were stably transfected with enhanced green fluorescent protein (EGFP)-tagged alpha- or beta-subunits of the epithelial Na+ channel (ENaC). Transfected RNA and proteins were both expressed in low abundance, similar to the endogenous levels of ENaC in native cells.

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Bibliographische Detailangaben
Veröffentlicht in:	American Journal of Physiology: Cell Physiology 2001-08, Vol.50 (2), p.C624-C632
Hauptverfasser:	BLAZER-YOST, Bonnie L, BUTTERWORTH, Michael, HARTMAN, Amy D, PARKER, Gretchen E, FALETTI, Carla J, ELS, Willem J, RHODES, Simon J
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell membranes. Ionic channels. Membrane pores Cell structures and functions Cells Fluorescence Fundamental and applied biological sciences. Psychology Insulin Molecular and cellular biology Proteins Sodium
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Beschreibung
Zusammenfassung:	A6 model renal epithelial cells were stably transfected with enhanced green fluorescent protein (EGFP)-tagged alpha- or beta-subunits of the epithelial Na+ channel (ENaC). Transfected RNA and proteins were both expressed in low abundance, similar to the endogenous levels of ENaC in native cells.
ISSN:	0363-6143 1522-1563