Fura-2 measurement of cytosolic free Ca(2plus) concentration in corpus allatum cells of larval Manduca sexta

Fura-2 measurement of cytosolic free Ca(2plus) concentration in corpus allatum cells of larval Manduca sexta

To determine whether differences in cystolic free calciumtwoplus in corpus allatum (CA) cells were associated with different levels of gland activity, methods were developed for dissociating CA cells with the retention of biosynthetic activity and for uptake by the dissociated cells of the fluoresce...

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Veröffentlicht in:Journal of experimental biology 1992-05, Vol.166, p.253
Hauptverfasser: Allen, C U, Herman, B, Granger, N A
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry
Calcium
Cellular biology
Fluorescence
Insects
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Zusammenfassung:To determine whether differences in cystolic free calciumtwoplus in corpus allatum (CA) cells were associated with different levels of gland activity, methods were developed for dissociating CA cells with the retention of biosynthetic activity and for uptake by the dissociated cells of the fluorescent calciumtwoplus indicator Fura-2.
ISSN:0022-0949
1477-9145