NMR-sensitive fluorinated and fluorescent intracellular calcium ion indicators with high dissociation constants

A new series of high-dissociation constant calcium2positive indicators has been developed to reduce perturbations due to buffering of transients, to carry out measurements in cells and organelles with high basal calcium2positive concentrations. Correction factors for the effects of intermediate exch...

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Veröffentlicht in:	American Journal of Physiology: Cell Physiology 1994-05, Vol.35 (5), p.C1313-C1322
Hauptverfasser:	LONDON, R. E, RHEE, C. K, MURPHY, E, GABEL, S, LEVY, L. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Anatomy & physiology Biological and medical sciences Calcium Diverse techniques Fundamental and applied biological sciences. Psychology Ions Molecular and cellular biology
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A new series of high-dissociation constant calcium2positive indicators has been developed to reduce perturbations due to buffering of transients, to carry out measurements in cells and organelles with high basal calcium2positive concentrations. Correction factors for the effects of intermediate exchange and for the effect of protonation and magnesium2positive complexation are discussed.
ISSN:	0363-6143 1522-1563