Mineralocorticoid and glucocorticoid receptor steroid binding and localization in colonic cells

Surface and crypt cells were sequentially isolated from rat distal colon by EDTA chelation and mechanical dissociation, and histologic examination, alkaline phosphatase activity and rates of (3H)leucine incorporation confirmed separation of surface from crypt cells. The data indicate that both surface and crypt cells are potentially responsive to mineralocorticoids (MRs) and glucocorticoids (GRs) and that both the MR and GR require hormone for nuclear translocation.