Improvement in Photostability of Fluorescein by Lanthanide Ions Based on Energy Transfer-based Triplet State Quenching

Photobleaching of fluorophores is a big obstacle in their advanced application in various systems. Since many photobleaching pathways originate from the long-lifetime triplet (T1) state, shortening the lifetime of the T1 state is a rational strategy to enhance the photostability of the fluorophores. In this study, we observed that some lanthanide ions promoted the photostability of fluorophores by quenching the T1 state via the triplet-triplet energy transfer from the fluorophore to the lanthanide ions.