ANG II AT1 and AT2 receptors in developing kidney of normal microswine

To identify an appropriate model of human renin-angiotensin system (RAS) involvement in fetal origins of adult disease, Bagby et al quantitated renal ANG II AT1 and AT2 receptors (AT1R and AT2R, respectively) in fetal, neonatal, and adult microswine by autoradiography (125I-labeled [Sar1Ile8]ANG II+cold CGP-42112 for AT1R, 125I-CGP-42112 for AT2R) and by whole kidney radioligand binding. The developmental pattern of renal AT1R in microswine, like many species, exhibited a 10-fold increase postnatally, with maximal postnatal density in glomeruli and lower density AT1R in extraglomerular cortical and outer medullary sites.