Development of an Immunoenzyme Assay to Control the Total Content of Antibiotics of the Fluoroquinolone Group in Milk

A method for preparing antibodies that specifically recognize antibiotics of the fluoroquinolone group with the same radical in the first position of the quinolone nucleus is proposed. The specificity of rabbit antisera prepared at different cycles of immunization by changing the structure of the ha...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Applied biochemistry and microbiology 2019-09, Vol.55 (5), p.563-569
Hauptverfasser: Shanin, I. A., Zvereva, E. A., Eremin, S. A., Sviridov, O. V., Zherdev, A. V., Dzantiev, B. B.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A method for preparing antibodies that specifically recognize antibiotics of the fluoroquinolone group with the same radical in the first position of the quinolone nucleus is proposed. The specificity of rabbit antisera prepared at different cycles of immunization by changing the structure of the hapten in the composition of immunogens was characterized. The selected antibodies provided a group-specific analysis of 16 representatives of fluoroquinolones, including a combination of the following compounds that are controlled in animal products: ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, and enrofloxacin. Using these antibodies, an indirect competitive enzyme-linked immunosorbent assay was developed that was characterized by a detection limit of ciprofloxacin of 0.2 ng/mL and a duration of 2 h. The assay was approbated for the detection of fluoroquinolones in milk.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683819050132