Nitric oxide reduces energy supply by direct action on the respiratory chain in isolated cardiomyocytes

To investigate the effecto f nitric oxide (NO) on cardiac energy metabolism, isolated cardiomyocytes of Wistar rats were incubated in an Oxystate system oat a constant ambient PO2 (25 mmHg) and oxygen consumption (VO2); free intracellular Ca2+ (fura 2), free cytosolic adenosine [S-adenosylhomocystei...

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Veröffentlicht in:	American journal of physiology. Heart and circulatory physiology 2001-05, Vol.49 (5), p.H2350-H2356
Hauptverfasser:	STUMPE, Thomas, DECKING, Ulrich K. M, SCHRADER, Jürgen
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Calcium Enzymes Fundamental and applied biological sciences. Psychology Gases Heart Oxygen Respiratory system Vertebrates: cardiovascular system
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Beschreibung
Zusammenfassung:	To investigate the effecto f nitric oxide (NO) on cardiac energy metabolism, isolated cardiomyocytes of Wistar rats were incubated in an Oxystate system oat a constant ambient PO2 (25 mmHg) and oxygen consumption (VO2); free intracellular Ca2+ (fura 2), free cytosolic adenosine [S-adenosylhomocysteine (SAH) medthod], and mitochondrila NADH (autofluorescence) were measured after application of the NO donor morpholinosydnonimine (SIN-1).
ISSN:	0363-6135 1522-1539