Anti-inflammatory and cytotoxic activities of constituents isolated from the fruits of Ziziphus jujuba var. inermis Rehder

Three new sesquilignans, zijusesquilignans A-C (1–3), together with fifteen known compounds (4–18), were isolated from fruits of Ziziphus jujuba var. inermis Rehder (Rhamnaceae). Their chemical structures were established using spectroscopic analyses including 1D- and 2D-NMR, HR-EIMS, and ECD spectra. These compounds were assessed for their inhibitory effects on nitric oxide (NO) production. Of these compounds, 1–3 and 17 displayed inhibitory effects on NO production, with IC50 values ranging from 18.1 to 66.4 μM. Pretreatment with 1 and 17 significantly suppressed LPS-induced expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein in cells. Moreover, compounds 1–3, 7, 9, and 17 exhibited cytotoxic activities against three human tumor cell lines, with IC50 values ranging from 8.4 to 44.9 μM. [Display omitted]